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PMID: 4560695 Published · ppublish English Journal Article

Regions of single-stranded DNA in the growing points of replicating bacteriophage T7 chromosomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 9 ·1972-09-00 ·Pages 2682-6

Wolfson J, Dressler D

Abstract

In partially replicated T7 chromosomes, the points where parental strands are separating and new DNA is being synthesized can be seen in the electron microscope to contain regions of single-stranded template DNA. The single-stranded regions are located on only one of the two daughter arms of the replicating chromosome. Inman and Schnös observed such single-stranded regions in 50% of the growing points of replicating lambda DNA, and, as reported in this paper, we find them in about 85% of the growing points of T7 DNA. Both studies support the conclusion that DNA synthesis involves the direct elongation of one daughter strand in the growing point. Evidently, this elongation is accompanied by the unwinding of the parental double helix to expose a region of single-stranded DNA which is then converted to the duplex state by a discontinuous mechanism involving the synthesis of DNA fragments.

MeSH Terms
Centrifugation, Density Gradient Chromosomes Coliphages DNA Replication DNA, Single-Stranded/biosynthesis DNA, Viral/biosynthesis Escherichia coli/metabolism Microscopy, Electron Models, Structural Nitrogen Isotopes Templates, Genetic
Chemicals
DNA, Single-Stranded DNA, Viral Nitrogen Isotopes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wolfson J
Dressler D
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.9.2682
Published
1972-09-00
Pages
2682-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427016
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.9.2682 PMC Full Text View journal details
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