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PMID: 3488209 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

T3 delta pre-mRNA is transcribed from a non-TATA promoter and is alternatively spliced in human T cells.

The EMBO journal ·Vol. 5 ·No. 6 ·1986-06-00 ·Pages 1245-52

Tunnacliffe A, Sims JE, Rabbitts TH

Abstract

The human T-cell antigen receptor-associated T3 complex consists of at least three polypeptides, gamma, delta and epsilon. cDNA clones for the delta-chain have recently been obtained and we have used such clones to isolate the T3 delta gene. The gene has been sequenced and comprises five exons, spread over approximately 3.7 kb of DNA. Transcription of the T3 delta gene is initiated from a non-TATA promoter. S1 mapping experiments and the sequence of a novel cDNA clone show that T3 delta mRNA exists in two forms in T cells. Alternative splicing of pre-mRNA sequences corresponding to the third exon of the T3 delta gene accounts for the two species of mRNA. A putative protein, produced by translation of the shorter mRNA, would lack a transmembrane region and might be secreted or associated with the outer surface of the cell.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/isolation & purification Genes Humans Nucleic Acid Precursors/genetics Promoter Regions, Genetic RNA Precursors RNA Splicing RNA, Messenger/genetics Receptors, Antigen, T-Cell/genetics T-Lymphocytes/immunology Transcription, Genetic
Chemicals
Nucleic Acid Precursors RNA Precursors RNA, Messenger Receptors, Antigen, T-Cell DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tunnacliffe A
Sims J E
Rabbitts T H
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49 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-06-00
Pages
1245-52
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1166934
Subset
IM
Databases
GENBANK
X03934
External Links
PubMed source PMC Full Text View journal details
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