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PMID: 3487086 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure of a bovine gene for P-450c21 (steroid 21-hydroxylase) defines a novel cytochrome P-450 gene family.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 12 ·1986-06-00 ·Pages 4243-7

Chung BC, Matteson KJ, Miller WL

Abstract

P-450c21, a cytochrome P-450 enzyme [steroid 21-monooxygenase (steroid 21-hydroxylase), EC 1.14.99.10], mediates the 21-hydroxylation of glucocorticoid and mineralocorticoid hormones in the adrenal gland. The complete sequence of a bovine P-450c21 gene shows it is 3447 base pairs long and contains 10 exons. The intron/exon organization and encoded amino acid sequence indicate that P-450c21 represents a unique family of genes in the P-450 gene superfamily. Primer extension and S1 nuclease protection experiments identified several cap sites for initiation of transcription; the principal cap site produces mRNA with a 5' untranslated region only 11 bases long. S1 nuclease protection experiments confirm that P-450c21 is actively expressed in the adrenal and the testis, an organ not known to secrete 21-hydroxylated steroids.

MeSH Terms
Amino Acid Sequence Animals Cattle Cytochrome P-450 Enzyme System/genetics Gene Expression Regulation Genes Glucocorticoids/physiology Sequence Homology, Nucleic Acid Steroid 21-Hydroxylase/genetics Steroid Hydroxylases/genetics
Chemicals
Glucocorticoids Cytochrome P-450 Enzyme System Steroid Hydroxylases Steroid 21-Hydroxylase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chung B C
Matteson K J
Miller W L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.12.4243
Published
1986-06-00
Pages
4243-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323708
Subset
IM
Grants
NICHD NIH HHS · HD16047 · United States
Databases
GENBANK
M11267, M13545
External Links
PubMed source DOI: 10.1073/pnas.83.12.4243 PMC Full Text View journal details
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