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PMID: 2999780 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of murine 21-hydroxylase in mouse adrenal glands and in transfected Y1 adrenocortical tumor cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 23 ·1985-12-00 ·Pages 7860-4

Parker KL, Chaplin DD, Wong M, Seidman JG, Smith JA, Schimmer BP

Abstract

The S region of the murine major histocompatibility complex contains two structurally related genes (21-OHase A and 21-OHase B) that encode 21-hydroxylase (21-OHase), an enzyme essential for the synthesis of adrenal steroids. Expression of these two genes has been analyzed by using oligonucleotide probes specific for the 21-OHase A and B genes and by DNA-mediated gene transfer. Hybridization of the oligonucleotides to blots of BALB/c adrenal RNA demonstrated that all 21-OHase mRNA is derived from the 21-OHase A gene. Cosmids bearing either the 21-OHase A or B gene were introduced into Y1 adrenocortical tumor cells by cotransfection with pSV2-neo. Cells transfected with the 21-OHase A gene expressed 21-OHase as determined by steroid metabolism and by RNA blot hybridization; 21-OHase transcripts were not detected in parent Y1 cells or in cells transfected with the 21-OHase B gene. Treatment of 21-OHase A transfectants with adrenocorticotropin increased 21-OHase mRNA levels by up to 10-fold, thus mimicking the observed effect of this hormone on 21-OHase levels in primary adrenal cultures. The regulated expression of the 21-OHase A gene in transfected Y1 cells should provide a useful system for the investigation of factors controlling the adrenal-specific regulation of 21-OHase activity.

MeSH Terms
Adrenal Cortex Neoplasms/genetics Adrenal Glands/physiology Adrenocorticotropic Hormone/physiology Animals Cloning, Molecular Gene Expression Regulation Macromolecular Substances Mice RNA, Messenger/genetics Steroid 21-Hydroxylase/genetics Steroid Hydroxylases/genetics Transcription, Genetic Transfection
Chemicals
Macromolecular Substances RNA, Messenger Adrenocorticotropic Hormone Steroid Hydroxylases Steroid 21-Hydroxylase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Parker K L
Chaplin D D
Wong M
Seidman J G
Smith J A
Schimmer B P
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.23.7860
Published
1985-12-00
Pages
7860-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390869
Subset
IM
Grants
NIAID NIH HHS · AI-18436 · United States
NIAID NIH HHS · AI-19148 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.23.7860 PMC Full Text View journal details
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