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PMID: 3460083 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nucleotide sequences of mRNAs encoding Epstein-Barr virus nuclear proteins: a probable transcriptional initiation site.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 14 ·1986-07-00 ·Pages 5096-100

Sample J, Hummel M, Braun D, Birkenbach M, Kieff E

Abstract

Three cDNA clones of the second Epstein-Barr virus nuclear antigen (EBNA2) mRNA and two of the EBNA1 mRNA were analyzed. Two EBNA2 clones begin 42 bases 3' to a promoter in the Epstein-Barr virus long internal repeat, which is likely to be the EBNA2 promoter. Surprisingly, the first splice creates an AUG at the beginning of the first of two nonoverlapping open reading frames. The second open reading frame encodes EBNA2. Two incomplete EBNA1 mRNA cDNA clones begin with parts of two of the EBNA2 exons and contain two other exons that map 19 and 59 kilobases 3' to the EBNA2 coding domain. The 3' exon of this mRNA encodes EBNA1. A model for regulation of transcription of these RNAs is presented.

MeSH Terms
Antigens, Viral/genetics Base Sequence DNA/analysis Epstein-Barr Virus Nuclear Antigens Genes, Viral Models, Genetic Promoter Regions, Genetic RNA, Messenger/genetics RNA, Viral/genetics Transcription, Genetic
Chemicals
Antigens, Viral Epstein-Barr Virus Nuclear Antigens RNA, Messenger RNA, Viral DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sample J
Hummel M
Braun D
Birkenbach M
Kieff E
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54 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.14.5096
Published
1986-07-00
Pages
5096-100
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323897
Subset
IM
Grants
NCI NIH HHS · CA 07967 · United States
NCI NIH HHS · CA 17281 · United States
NCI NIH HHS · CA 19264 · United States
Databases
GENBANK
K03489, M13940, M13941
External Links
PubMed source DOI: 10.1073/pnas.83.14.5096 PMC Full Text View journal details
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