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PMID: 3005654 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Orientation and patching of the latent infection membrane protein encoded by Epstein-Barr virus.

Journal of virology ·Vol. 58 ·No. 1 ·1986-04-00 ·Pages 233-7

Liebowitz D, Wang D, Kieff E

Abstract

Epstein-Barr virus is known to encode three nuclear proteins and one membrane protein (LMP) in latently infected growth-transformed cells. Studies of the plasma membrane localization and orientation of LMP by protease digestion of live cells and by immunofluorescence indicated the following. (i) At least 30% of LMP is in the plasma membrane, as opposed to other cytoplasmic membranes. (ii) A small LMP domain which corresponds to a previously proposed outer reverse turn between the first two transmembrane domains is exposed on the outer cell surface (and two other proposed outer-reverse-turn domains may be exposed), whereas all or almost all of the rest of the protein is not exposed on the outer cell surface. (iii) LMP is present in patches in the cell plasma membrane.

MeSH Terms
Antigens, Viral/immunology Cell Compartmentation Cell Membrane/immunology,ultrastructure Cells, Cultured Herpesvirus 4, Human/immunology Membrane Proteins/immunology,metabolism Peptide Hydrolases/metabolism Protein Conformation Viral Proteins/immunology,metabolism
Chemicals
Antigens, Viral Membrane Proteins Viral Proteins Peptide Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Liebowitz D
Wang D
Kieff E
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29 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.58.1.233-237.1986
Published
1986-04-00
Pages
233-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252901
Subset
IM
Grants
NCI NIH HHS · CA 17281 · United States
NCI NIH HHS · CA 19264 · United States
NIGMS NIH HHS · GM 07281 · United States
External Links
PubMed source DOI: 10.1128/JVI.58.1.233-237.1986 PMC Full Text View journal details
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