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PMID: 2991591 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Epstein-Barr virus-encoded protein found in plasma membranes of transformed cells.

Journal of virology ·Vol. 55 ·No. 3 ·1985-09-00 ·Pages 710-20

Mann KP, Staunton D, Thorley-Lawson DA

Abstract

We have developed monoclonal antibodies to a 63,000-molecular-weight protein (p63) which is the product of the most abundant messenger RNA in Epstein-Barr virus-transformed cells and shown that the protein is associated specifically with plasma membranes. It was also found to be associated with the other membrane fractions and was found in all Epstein-Barr virus-transformed cells tested. In addition, p63 was present in virions, resulting in transient, early appearance in newly infected cells. Newly synthesized p63 was detected at the time cells underwent blast transformation (48 to 72 h postinfection). The possible role of this protein in transformation and as a target for cell-mediated cytotoxicity is discussed.

MeSH Terms
Antibodies, Monoclonal Cell Membrane/analysis,microbiology Cell Transformation, Viral Fluorescent Antibody Technique Herpesvirus 4, Human/genetics Humans Molecular Weight Transfection Viral Proteins/analysis Virion/analysis
Chemicals
Antibodies, Monoclonal Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mann K P
Staunton D
Thorley-Lawson D A
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47 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.55.3.710-720.1985
Published
1985-09-00
Pages
710-20
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255052
Subset
IM
Grants
NIAID NIH HHS · AI00549 · United States
NIAID NIH HHS · AI15310 · United States
NCI NIH HHS · CA28737 · United States
External Links
PubMed source DOI: 10.1128/JVI.55.3.710-720.1985 PMC Full Text View journal details
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