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PMID: 3422422 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Differential expression of insulin-like growth factor genes in rat central nervous system.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 1 ·1988-01-00 ·Pages 265-9

Rotwein P, Burgess SK, Milbrandt JD, Krause JE

Abstract

A sensitive solution-hybridization assay was used to investigate the expression of genes encoding insulin-like growth factors I and II (IGF-I and -II) in the rat central nervous system (CNS). mRNAs for both IGFs are synthesized throughout the CNS of adult rats but exhibit distinct regional differences for each growth factor. IGF-I mRNA is 8-10 times more abundant in the cervical-thoracic spinal cord and in the olfactory bulb than in whole brain and is enriched 3-fold in the midbrain and cerebellum. IGF-II mRNA is minimally enriched in the medulla-pons and cerebellum but is 3-5 times less abundant in the midbrain and corpus striatum than in total brain. During CNS development the content of IGF-I and IGF-II mRNAs is highest at embryonic day 14 and declines by a factor of 3-4 at birth, to values found in adult brain. Embryonic neurons and glia synthesize IGF-I mRNA during short-term cell culture; only glia produce IGF-II mRNA. These observations show that IGF-I and IGF-II are differentially expressed in the developing and adult CNS and suggest that each growth factor may play a unique role in the mammalian nervous system.

MeSH Terms
Aging Animals Brain/embryology,growth & development,metabolism Embryo, Mammalian Embryonic and Fetal Development Exons Genes Insulin-Like Growth Factor I/genetics Insulin-Like Growth Factor II/genetics RNA, Messenger/analysis,genetics Rats Rats, Inbred Strains Somatomedins/genetics Transcription, Genetic
Chemicals
RNA, Messenger Somatomedins Insulin-Like Growth Factor I Insulin-Like Growth Factor II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rotwein P
Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.
Burgess S K
Milbrandt J D
Krause J E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.1.265
Published
1988-01-00
Pages
265-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279525
Subset
IM
Grants
NIDDK NIH HHS · DK37449 · United States
NINDS NIH HHS · NS01018 · United States
NINDS NIH HHS · NS21937 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.1.265 PMC Full Text View journal details
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