Home LiteratureArticle Details
PMID: 3460078 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of somatomedins from human fetal brain: identification of a variant form of insulin-like growth factor I.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 13 ·1986-07-00 ·Pages 4904-7

Sara VR, Carlsson-Skwirut C, Andersson C, Hall E, Sjögren B, Holmgren A, Jörnvall H

Abstract

A fetal form of somatomedin (insulin-like growth factor) that crossreacts in the fetal brain radioreceptor assay has been proposed to exist in humans. Using this assay to monitor activity during purification, we have isolated a variant form of insulin-like growth factor I (IGF-I) from human fetal brain tissue. The variant IGF-I showed potent crossreaction in the fetal brain radioreceptor assay and stimulated DNA synthesis in fetal brain cells in vitro. Structural analysis revealed the variant IGF-I to have a truncated NH2-terminal region compared to IGF-I isolated from serum. An additional peptide, which displayed less potent crossreaction in the fetal brain radioreceptor assay, was also isolated from the human fetal brain. Partial amino acid sequence analysis revealed identity to insulin-like growth factor II.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Brain/embryology Brain Chemistry Chromatography, High Pressure Liquid Cytosol/analysis Humans Insulin-Like Growth Factor I/isolation & purification Radioligand Assay Somatomedins/isolation & purification
Chemicals
Amino Acids Somatomedins Insulin-Like Growth Factor I
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Sara V R
Carlsson-Skwirut C
Andersson C
Hall E
Sjögren B
Holmgren A
Jörnvall H
References (16)
16 references, click to expand
  1. A new spectrophotometric assay for protein in cell extracts.
    Anal Biochem. 1977 Oct;82(2):362-71 PMID: 20815
  2. The influence of purified somatomedins and insulin on foetal rat brain DNA synthesis in vitro.
    Acta Physiol Scand. 1985 Oct;125(2):305-8 PMID: 3907275
  3. Primary structure of human insulin-like growth factor II.
    FEBS Lett. 1978 May 15;89(2):283-6 PMID: 658418
  4. Immunoreactive somatomedin A in human serum.
    J Clin Endocrinol Metab. 1979 Feb;48(2):271-8 PMID: 218985
  5. Purification of somatomedin-C from human plasma: chemical and biological properties, partial sequence analysis, and relationship to other somatomedins.
    Biochemistry. 1980 Feb 19;19(4):790-7 PMID: 7188854
  6. Somatomedins and the fetus.
    Clin Obstet Gynecol. 1980 Sep;23(3):765-78 PMID: 6998626
  7. Human embryonic somatomedin.
    Proc Natl Acad Sci U S A. 1981 May;78(5):3175-9 PMID: 6942423
  8. Ontogenesis of somatomedin and insulin receptors in the human fetus.
    J Clin Invest. 1983 May;71(5):1084-94 PMID: 6304143
  9. Sequence of cDNA encoding human insulin-like growth factor I precursor.
    Nature. 1983 Dec 8-14;306(5943):609-11 PMID: 6358902
  10. Growth and somatomedins.
    Vitam Horm. 1983;40:175-233 PMID: 6324479
  11. The characterization of somatomedin A, isolated by microcomputer-controlled chromatography, reveals an apparent identity to insulin-like growth factor 1.
    Eur J Biochem. 1984 Aug 15;143(1):117-24 PMID: 6381052
  12. Nucleotide sequences of cDNAs encoding precursors of human insulin-like growth factor II (IGF-II) and an IGF-II variant.
    FEBS Lett. 1985 Jan 7;179(2):243-6 PMID: 3881277
  13. Insulin-like growth factor II (IGF II) in human brain: regional distribution of IGF II and of higher molecular mass forms.
    Proc Natl Acad Sci U S A. 1985 Apr;82(7):2153-7 PMID: 3885223
  14. Amino acid sequence of a variant pro-form of insulin-like growth factor II.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3169-72 PMID: 3889903
  15. Expression of insulin-like growth factor-II transcripts in Wilms' tumour.
    Nature. 1985 Sep 19-25;317(6034):258-60 PMID: 2995817
  16. The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin.
    J Biol Chem. 1978 Apr 25;253(8):2769-76 PMID: 632300
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.13.4904
Published
1986-07-00
Pages
4904-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323852
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.13.4904 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2