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PMID: 3889903 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Amino acid sequence of a variant pro-form of insulin-like growth factor II.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 10 ·1985-05-00 ·Pages 3169-72

Zumstein PP, Lüthi C, Humbel RE

Abstract

Human serum contains, in addition to the "classical" 7.5-kDa insulin-like growth factors (IGFs) I and II, small amounts of larger IGF-II. A 10-kDa IGF-II was isolated by gel filtration, immunoaffinity chromatography, and reversed-phase HPLC. Upon amino acid sequence determination, a substitution of Cys-Gly-Asp for Ser-33 was found as well as a COOH-terminal extension of 21 residues (E peptide). These sequence differences suggest that 10-kDa IGF-II is a precursor of a variant IGF-II. Since the substitution is not located at a known intron/exon hinge region, the finding of this variant IGF-II is evidence for the presence of more than one gene for IGF-II.

MeSH Terms
Amino Acid Sequence Humans Insulin/blood,isolation & purification Molecular Weight Peptide Fragments/analysis Peptides/blood,isolation & purification Protein Precursors/isolation & purification Radioimmunoassay Somatomedins/blood,isolation & purification
Chemicals
Insulin Peptide Fragments Peptides Protein Precursors Somatomedins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zumstein P P
Lüthi C
Humbel R E
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20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.10.3169
Published
1985-05-00
Pages
3169-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397736
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.10.3169 PMC Full Text View journal details
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