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PMID: 3889836 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Developmental and tissue-specific expression of a family of transcripts related to rat insulin-like growth factor II mRNA.

Nucleic acids research ·Vol. 13 ·No. 4 ·1985-02-25 ·Pages 1119-34

Soares MB, Ishii DN, Efstratiadis A

Abstract

We have constructed a cDNA library from the mRNA of a rat liver cell line (BRL-3A) and characterized cDNA clones encoding the protein precursor of the rat insulin-like growth factor II (pre-pro-rIGF-II). This precursor, inferred from the nucleotide sequence, consists of a signal peptide, the rIGF-II sequence, and a trailer polypeptide of unknown significance. The characterized cDNA sequence (1016 nt) is part of a 3.4 kb mRNA species. Northern analysis reveals that a probe containing the extreme 5' noncoding region hybridizes to a second RNA (1.6 kb), while a probe corresponding to the 5' noncoding region proximal to the coding region hybridizes to two other RNA species (1.75 and 1.1 kb). All four RNAs are differentially expressed in all of the neonatal tissues that were examined, while the 3.4 kb pre-pro-rIGF-II mRNA and the 1.1 kb transcript are absent from adult tissues.

MeSH Terms
Amino Acid Sequence Animals Animals, Newborn Base Sequence Cloning, Molecular DNA/genetics Gene Expression Regulation Insulin/genetics Peptides/genetics Protein Precursors/genetics RNA Processing, Post-Transcriptional RNA, Messenger/genetics Rats Somatomedins/genetics Tissue Distribution Transcription, Genetic
Chemicals
Insulin Peptides Protein Precursors RNA, Messenger Somatomedins DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Soares M B
Ishii D N
Efstratiadis A
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31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.4.1119
Published
1985-02-25
Pages
1119-34
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341060
Subset
IM
Databases
GENBANK
X02213
External Links
PubMed source DOI: 10.1093/nar/13.4.1119 PMC Full Text View journal details
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