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PMID: 3375068 Published · ppublish English Journal Article

Specific binding of cruciform DNA structures by a protein from human extracts.

Nucleic acids research ·Vol. 16 ·No. 9 ·1988-05-11 ·Pages 3603-16

Elborough KM, West SC

Abstract

A gel electrophoresis binding assay has been used to probe extracts from cultured human lymphoblasts for proteins that bind cruciform structures in duplex DNA. Proteins have been detected that form complexes with synthetic X- and Y-junctions. Several lines of evidence suggest that binding is specific for DNA structure rather than sequence: (1) X- and Y-structures were bound whereas linear duplexes containing identical DNA sequences were not, (2) Binding occurred with equal efficiency to two X-junctions that were constructed from DNA strands of different sequence, (3) One X-junction successfully competed with another for binding whereas linear duplex DNA did not; and (4) protein-DNA complexes were observed at probe:non-specific competitor DNA ratios of 1:10,000.

MeSH Terms
Base Sequence Cell Line DNA/metabolism DNA Polymerase I/metabolism DNA-Binding Proteins/isolation & purification,metabolism Humans Molecular Sequence Data Nucleic Acid Conformation Oligodeoxyribonucleotides/chemical synthesis,metabolism Protein Binding
Chemicals
DNA-Binding Proteins Oligodeoxyribonucleotides DNA DNA Polymerase I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Elborough K M
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK.
West S C
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.9.3603
Published
1988-05-11
Pages
3603-16
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336545
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.9.3603 PMC Full Text View journal details
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