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PMID: 6950379 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Replication of linear mitochondrial DNA from Paramecium: sequence and structure of the initiation-end crosslink.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 12 ·1981-12-00 ·Pages 7341-5

Pritchard AE, Cummings DJ

Abstract

Replication of the 14-micrometer linear Paramecium mitochondrial DNA is initiated by a crosslinking of the duplex strands at the initiation end of the molecule. As a consequence of the crosslink, a head-to-head dimer molecule, or palindrome, is a replicative intermediate. The central region of the dimer molecules of two species was cloned and sequenced, In the distal regions, the sequence is palindromic as expected; however, in the central region, there is a nonpalindromic sequence that is rich in A + T and contains direct tandem repeats. It is proposed that the nonpalindromic sequence constitutes the crosslink. Implications for the replication scheme are discussed. The model is unlike any other for a linear DNA.

MeSH Terms
Animals Base Sequence DNA Replication DNA, Mitochondrial/genetics Paramecium/genetics
Chemicals
DNA, Mitochondrial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pritchard A E
Cummings D J
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.12.7341
Published
1981-12-00
Pages
7341-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349262
Subset
IM
Grants
NIGMS NIH HHS · GM 21948 · United States
Databases
GENBANK
J01432, J01433
External Links
PubMed source DOI: 10.1073/pnas.78.12.7341 PMC Full Text View journal details
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