Effects and virulences of recombinant vaccinia viruses derived from attenuated strains that express the human T-cell leukemia virus type I envelope gene.

Journal of virology ·Vol. 62 ·No. 12 ·1988-12-00 ·Pages 4474-80

Shida H, Hinuma Y, Hatanaka M, Morita M, Kidokoro M, Suzuki K, Maruyama T, Takahashi-Nishimaki F, Sugimoto M, Kitamura R

Abstract

We constructed recombinant vaccinia viruses (RVVs) that expressed human T-cell leukemia virus type I (HTLV-I) envelope glycoproteins by using attenuated vaccinia viruses (VVs) which have much lower neurovirulence than the WR strain that is extensively used as a vector. The RVV produced from the LC16mO strain, one of the attenuated VVs, elicited a high titer of anti-HTLV-I antibody in rabbits and protected them against HTLV-I infection. The env gene was inserted into the VV hemagglutinin gene. The resultant inactivation of the hemagglutinin gene led to the attenuation of VVs, but the extent of their attenuation depended on the VV strain. The propagation of LC16mO and its RVV in rabbit brain was poorer than that of LO-1, a cloned derivative of Lister strain, and its RVV, although LC16mO replicated in other organs better than did LO-1. Taken together, these results suggest that LC16mO is a good candidate as a vector for vaccination of humans.

MeSH Terms

Animals Brain/microbiology Cell Line Gene Expression Regulation Genes, Viral Glycoproteins/biosynthesis,genetics,immunology HTLV-I Infections/prevention & control Hemagglutinins, Viral/genetics Human T-lymphotropic virus 1/genetics,immunology Male Mice Precipitin Tests Rabbits Vaccines Vaccines, Attenuated Vaccines, Synthetic Vaccinia virus/genetics,growth & development,immunology,pathogenicity Viral Envelope Proteins/biosynthesis,genetics,immunology Viral Vaccines Virulence

Chemicals

Glycoproteins Hemagglutinins, Viral Vaccines Vaccines, Attenuated Vaccines, Synthetic Viral Envelope Proteins Viral Vaccines

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Shida H

Institute for Virus Research, Kyoto University, Japan.

Hinuma Y

Hatanaka M

Morita M

Kidokoro M

Suzuki K

Maruyama T

Takahashi-Nishimaki F

Sugimoto M

Kitamura R

References (22)

22 references, click to expand

Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera.
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6476-80 PMID: 7031654
The purification fo four strains of poxvirus.
Virology. 1962 Sep;18:9-18 PMID: 14036977
Vaccinia virus: a selectable eukaryotic cloning and expression vector.
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7415-9 PMID: 6296831
Infectious vaccinia virus recombinants that express hepatitis B virus surface antigen.
Nature. 1983 Apr 7;302(5908):490-5 PMID: 6835382
Analysis of the hemagglutinin glycoprotein from mutants of vaccinia virus that accumulates on the nuclear envelope.
Cell. 1983 Jun;33(2):423-34 PMID: 6861204
Construction and characterization of an infectious vaccinia virus recombinant that expresses the influenza hemagglutinin gene and induces resistance to influenza virus infection in hamsters.
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7155-9 PMID: 6580632
Construction of live vaccines using genetically engineered poxviruses: biological activity of vaccinia virus recombinants expressing the hepatitis B virus surface antigen and the herpes simplex virus glycoprotein D.
Proc Natl Acad Sci U S A. 1984 Jan;81(1):193-7 PMID: 6320164
Monoclonal integration of human T-cell leukemia provirus in all primary tumors of adult T-cell leukemia suggests causative role of human T-cell leukemia virus in the disease.
Proc Natl Acad Sci U S A. 1984 Apr;81(8):2534-7 PMID: 6326131
Identification of gag and env gene products of human T-cell leukemia virus (HTLV).
Virology. 1984 Jul 30;136(2):338-47 PMID: 6087548
Cell surface phenotypes and expression of viral antigens of various human cell lines carrying human T-cell leukemia virus.
Int J Cancer. 1984 Aug 15;34(2):221-8 PMID: 6088403
Live recombinant vaccinia virus protects chimpanzees against hepatitis B.
Nature. 1984 Sep 6-11;311(5981):67-9 PMID: 6472464
Expression of rabies virus glycoprotein from a recombinant vaccinia virus.
Nature. 1984 Nov 8-14;312(5990):163-6 PMID: 6548799
In vitro mutagenesis of the promoter region for a vaccinia virus gene: evidence for tandem early and late regulatory signals.
J Virol. 1985 Apr;54(1):30-7 PMID: 3973982
Vaccinia virus recombinant expressing herpes simplex virus type 1 glycoprotein D prevents latent herpes in mice.
Science. 1985 May 10;228(4700):737-40 PMID: 2986288
Decreased virulence of recombinant vaccinia virus expression vectors is associated with a thymidine kinase-negative phenotype.
Nature. 1985 Oct 31-Nov 6;317(6040):813-5 PMID: 4058585
Resistance to human respiratory syncytial virus (RSV) infection induced by immunization of cotton rats with a recombinant vaccinia virus expressing the RSV G glycoprotein.
Proc Natl Acad Sci U S A. 1986 Mar;83(6):1906-10 PMID: 3513191
Nucleotide sequence of the vaccinia virus hemagglutinin gene.
Virology. 1986 Apr 30;150(2):451-62 PMID: 3008418
Genetic analysis of vaccinia virus Lister strain and its attenuated mutant LC16m8: production of intermediate variants by homologous recombination.
J Gen Virol. 1987 Oct;68 ( Pt 10):2705-10 PMID: 3668510
Prevention of vaccinia virus infection in immunodeficient mice by vector-directed IL-2 expression.
Nature. 1987 Nov 19-25;330(6145):259-62 PMID: 3118219
Cloning and characterization of the gene encoding the major protein of the A-type inclusion body of cowpox virus.
J Gen Virol. 1988 Jan;69 ( Pt 1):35-47 PMID: 2826668
Effect of the recombinant vaccinia viruses that express HTLV-I envelope gene on HTLV-I infection.
EMBO J. 1987 Nov;6(11):3379-84 PMID: 2828027
Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus.
Proc Natl Acad Sci U S A. 1982 Aug;79(16):4927-31 PMID: 6289324

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.62.12.4474-4480.1988

Published

1988-12-00

Pages

4474-80

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC253556

Subset

External Links

PubMed source DOI: 10.1128/JVI.62.12.4474-4480.1988 PMC Full Text View journal details

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Effects and virulences of recombinant vaccinia viruses derived from attenuated strains that express the human T-cell leukemia virus type I envelope gene.

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