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PMID: 3155715 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Escherichia coli supH suppressor: temperature-sensitive missense suppression caused by an anticodon change in tRNASer2.

Journal of bacteriology ·Vol. 161 ·No. 1 ·1985-01-00 ·Pages 207-11

Thorbjarnardóttir S, Uemura H, Dingermann T, Rafnar T, Thorsteinsdóttir S, Söll D, Eggertsson G

Abstract

We describe the cloning and the DNA sequence of the Escherichia coli supH missense suppressor and of the supD60(Am) suppressor genes. supH is a mutant form of serU which codes for tRNASer2. The supH coding sequence differs from the wild-type sequence by a single nucleotide change which corresponds to the middle position of the anticodon. The CGA anticodon of wild-type tRNA and CUA anticodon of supD tRNA is changed to CAA in supH tRNA, which is expected to recognize the UUG leucine codon. We propose that the supH suppressor causes the insertion of serine in response to this codon. The temperature sensitivity caused by supH may be due to a conformation of the CAA anticodon in the supH tRNASer that is slightly different than that in the corresponding tRNALeu species.

MeSH Terms
Anticodon/genetics Bacteriophage lambda/genetics Base Sequence Cloning, Molecular Escherichia coli/genetics Mutation RNA, Transfer, Amino Acyl/genetics Suppression, Genetic Temperature Transduction, Genetic
Chemicals
Anticodon RNA, Transfer, Amino Acyl
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Thorbjarnardóttir S
Uemura H
Dingermann T
Rafnar T
Thorsteinsdóttir S
Söll D
Eggertsson G
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42 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.161.1.207-211.1985
Published
1985-01-00
Pages
207-11
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214857
Subset
IM
Databases
GENBANK
M10746, M10748
External Links
PubMed source DOI: 10.1128/jb.161.1.207-211.1985 PMC Full Text View journal details
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