Home LiteratureArticle Details
PMID: 353502 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mutations determining generalized resistance to aminoglycoside antibiotics in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 161 ·No. 1 ·1978-04-25 ·Pages 89-98

Thorbjarnardóttir SH, Magnúsdóttir RA, Eggertsson G

Abstract

Mutations conferring resistance to low levels of kanamycin in Escherichia coli have been mapped at 3 locations: the unc locus (min. 83), a locus we have designated kanA (MIN. 72), close to strA (rpsL), and a locus at min. 86.5 previously discovered by Plate (1976) that we have designated ecfB. The unc and ecfB mutations are associated with defects in energy metabolism, while mutations at kanA may be in the gene coding for ribosomal protein S12 (rpsL). The three types of mutations cause cross resistance to a number of different aminoglycoside antibiotics and the effects of the mutations are cumulative in combination.

MeSH Terms
Ampicillin/pharmacology Chloramphenicol/pharmacology Chromosome Mapping Chromosomes, Bacterial Dose-Response Relationship, Drug Escherichia coli/drug effects,genetics Escherichia coli Proteins Genes Kanamycin/pharmacology Penicillin Resistance Ribosomal Protein S9 Tetracycline/pharmacology Transduction, Genetic
Chemicals
Escherichia coli Proteins Ribosomal Protein S9 RpsI protein, E coli Kanamycin Chloramphenicol Ampicillin Tetracycline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Thorbjarnardóttir S H
Magnúsdóttir R A
Eggertsson G
References (35)
35 references, click to expand
  1. Alteration of ribosomal protein S17 by mutation linked to neamine resistance in Escherichia coli. I. General properties of neaA mutants.
    J Mol Biol. 1975 Dec 25;99(4):795-806 PMID: 765484
  2. Suppression of spectinomycin resistance in a mutant of Escherichia coli K-12.
    J Bacteriol. 1975 Jun;122(3):1103-8 PMID: 125262
  3. Mapping a cluster of ribosomal genes in Escherichia coli.
    Mol Gen Genet. 1974;133(4):317-27 PMID: 4280012
  4. Activity of ribosomes from kanamycin-resistant E. coli.
    Biochem Biophys Res Commun. 1964 Jun 15;16(3):216-20 PMID: 5332848
  5. Mapping of neamine resistance: identification to two genetic loci, nea A and nea B.
    Mol Gen Genet. 1974 Jun 27;130(4):321-6 PMID: 4604727
  6. Cooperative control of translation fidelity by ribosomal proteins in Escherichia coli. I. Properties of ribosomal mutants whose resistance to neamine is the cumulative effect of two distinct mutations.
    Mol Gen Genet. 1975 Dec 23;142(1):19-33 PMID: 765735
  7. Mechanisms of antibiotic resistance in bacteria.
    Annu Rev Biochem. 1973;42:471-506 PMID: 4581231
  8. Reconstitution of the energy-linked transhydrogenase activity in membranes from a mutant strain of Escherichia coli K12 lacking magnesium ion- or calcium ion-stimulated adenosine triphosphatase.
    Biochem J. 1973 Apr;132(4):689-95 PMID: 4269101
  9. Transposition of R factor genes to bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3628-32 PMID: 1059152
  10. A semi-quantitative treatment of missense and nonsense suppression in the strA and ram ribosomal mutants of Escherichia coli. Evaluation of some molecular parameters of translation in vivo.
    J Mol Biol. 1974 Apr 5;84(2):297-313 PMID: 4598370
  11. Isolation and characterization of mutants of Escherichia coli K12 resistant to the new aminoglycoside antibiotic, amikacin.
    J Gen Microbiol. 1976 Jun;94(2):389-94 PMID: 781182
  12. Use of neomycin in the isolation of mutants blocked in energy conservation in Escherichia coli.
    J Bacteriol. 1972 Jul;111(1):287-9 PMID: 4273171
  13. Restoration of active transport in an Mg2+-adenosine triphosphatase-deficient mutant of Escherichia coli.
    J Bacteriol. 1973 Dec;116(3):1124-9 PMID: 4270946
  14. Hybridization between Escherichia coli and Shigella.
    J Bacteriol. 1957 Oct;74(4):461-76 PMID: 13475269
  15. Hemin-deficient mutants of Escherichia coli K-12.
    J Bacteriol. 1968 Aug;96(2):570-2 PMID: 4877132
  16. Mapping and complementation of three genes specifying 30S ribosomal components in Escherichia coli.
    J Bacteriol. 1968 Oct;96(4):1431-2 PMID: 4879566
  17. A mutant of Escherichia coli defective in the coupling of metabolic energy to active transport.
    Proc Natl Acad Sci U S A. 1974 Nov;71(11):4395-9 PMID: 4280071
  18. The energetics of bacterial active transport.
    Annu Rev Biochem. 1975;44:523-54 PMID: 237462
  19. Transduction of linked genetic characters of the host by bacteriophage P1.
    Virology. 1955 Jul;1(2):190-206 PMID: 13267987
  20. Coresistance to neomycin and kanamycin by mutations in an Escherichia coli locus that affects ribosomes.
    J Bacteriol. 1968 Sep;96(3):768-76 PMID: 4895052
  21. Mutant of Escherichia coli defective in response to colicin K and in active transport.
    J Bacteriol. 1976 Feb;125(2):467-74 PMID: 128554
  22. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  23. Binding of dihydrostreptomycin to Escherichia coli ribosomes: kinetics of the reaction.
    Antimicrob Agents Chemother. 1972 Oct;2(4):308-19 PMID: 4133237
  24. Gentamicin accumulation by sensitive strains of Escherichia coli and Pseudomonas aeruginosa.
    J Antibiot (Tokyo). 1975 Sep;28(9):696-703 PMID: 810469
  25. Alteration of ribosomal protein L6 in mutants of Escherichia coli resistant to gentamicin.
    Mol Gen Genet. 1977 Dec 14;158(1):47-54 PMID: 342908
  26. Kinetic proofreading: a new mechanism for reducing errors in biosynthetic processes requiring high specificity.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):4135-9 PMID: 4530290
  27. Bacterial respiration.
    Bacteriol Rev. 1977 Mar;41(1):47-99 PMID: 140652
  28. Gentamicin resistance in clinical-isolates of Pseudomonas aeruginosa associated with diminished gentamicin accumulation and no detectable enzymatic modification.
    J Antibiot (Tokyo). 1976 Jul;29(7):743-53 PMID: 821904
  29. Map positions and specificities of suppressor mutations in Escherichia coli K-12.
    Genetics. 1965 Aug;52(2):319-40 PMID: 5324068
  30. Properties of Escherichia coli mutants with alterations in Mg2+-adenosine triphosphatase.
    J Bacteriol. 1976 Oct;128(1):248-56 PMID: 135756
  31. Functional interdependence of ribosomal components of Escherichia coli.
    Proc Natl Acad Sci U S A. 1969 Jul;63(3):794-9 PMID: 4899876
  32. Chromosomal location of the structural gene for glycerol kinase in Escherichia coli.
    J Bacteriol. 1966 May;91(5):1763-6 PMID: 5327904
  33. Effects of membrane-energy mutations and cations on streptomycin and gentamicin accumulation by bacteria: a model for entry of streptomycin and gentamicin in susceptible and resistant bacteria.
    Antimicrob Agents Chemother. 1977 Aug;12(2):163-77 PMID: 143238
  34. Characterization of Escherichia coli mutant incapable of maintaining a transmembrane potential. MetC ecfts mutations.
    J Biol Chem. 1977 Jun 25;252(12):4056-67 PMID: 16923
  35. Localization of sensitivity to kanamycin and streptomycin in 30 S ribosomal proteins of Escherichia coli.
    J Antibiot (Tokyo). 1969 Dec;22(12):612-23 PMID: 4904480
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00266619
Published
1978-04-25
Pages
89-98
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00266619
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2