Home LiteratureArticle Details
PMID: 135756 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Properties of Escherichia coli mutants with alterations in Mg2+-adenosine triphosphatase.

Journal of bacteriology ·Vol. 128 ·No. 1 ·1976-10-00 ·Pages 248-56

Adler LW, Rosen BP

Abstract

A mutant Escherichia coli, selected for resistance to the antibiotic neomycin, was unable to utilize nonfermentable carbon sources for growth. Two strains were selected from this mutant on the basis of their ability to grow utilizing succinate as a carbon source. All three strains had approximately equal amounts of the Mg2+-adenosine triphosphatase (ATPase) (EC 3.6.1.3) protein, but the activity of the enzyme differed in each strain. The Mg2+-ATPase from each of the three strains lost activity upon solubilization and appeared to undergo rapid dissociation once solubilized. This dissociation is similar to that described for the wild type after cold exposure.

MeSH Terms
Adenosine Triphosphatases/analysis,isolation & purification,metabolism Cell Membrane/metabolism Chromosome Mapping Chromosomes, Bacterial Dicyclohexylcarbodiimide/pharmacology Drug Resistance, Microbial Escherichia coli/enzymology,metabolism Ethanol/pharmacology Immunodiffusion Immunoelectrophoresis Mutation Neomycin/pharmacology
Chemicals
Ethanol Dicyclohexylcarbodiimide Adenosine Triphosphatases Neomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Adler L W
Rosen B P
References (18)
18 references, click to expand
  1. ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS.
    Anal Biochem. 1965 Feb;10:358-61 PMID: 14302464
  2. A mutant of Escherichia coli with a defect in energy metabolism.
    J Gen Microbiol. 1972 May;70(3):507-15 PMID: 4556255
  3. The energetics of bacterial active transport.
    Annu Rev Biochem. 1975;44:523-54 PMID: 237462
  4. Oxidative phosphorylation in Escherichia coli K12. Mutations affecting magnesium ion- or calcium ion-stimulated adenosine triphosphatase.
    Biochem J. 1971 Aug;124(1):75-81 PMID: 4256722
  5. -Galactoside accumulation in a Mg 2+ -,Ca 2+ -activated ATPase deficient mutant of E.coli.
    Biochem Biophys Res Commun. 1972 Aug 7;48(3):544-51 PMID: 4261724
  6. Essential role of membrane ATPase or coupling factor for anaerobic growth and anaerobic active transport in Escherichia coli.
    Biochim Biophys Acta. 1973 Sep 26;314(3):267-75 PMID: 4270850
  7. The maintenance of the energized membrane state and its relation to active transport in Escherichia coli.
    Biochim Biophys Acta. 1975 Apr 14;387(1):23-36 PMID: 123782
  8. Energy transduction in Escherichia coli. The role of the Mg2+ATPase.
    J Biol Chem. 1975 Nov 10;250(21):8409-15 PMID: 127791
  9. ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI.
    J Biol Chem. 1964 Sep;239:3098-105 PMID: 14217902
  10. TOPOGRAPHY OF COTRANSDUCIBLE ARGININE MUTATIONS IN ESCHERICHIA COLI K-12.
    Genetics. 1965 Feb;51:167-79 PMID: 14292146
  11. Restoration of active transport in an Mg2+-adenosine triphosphatase-deficient mutant of Escherichia coli.
    J Bacteriol. 1973 Dec;116(3):1124-9 PMID: 4270946
  12. Coupling between energy conservation and active transport of serine in Escherichia coli.
    Biochim Biophys Acta. 1973 Oct 25;323(3):429-40 PMID: 4271263
  13. Use of neomycin in the isolation of mutants blocked in energy conservation in Escherichia coli.
    J Bacteriol. 1972 Jul;111(1):287-9 PMID: 4273171
  14. Replacement of a phosphoenolpyruvate-dependent phosphotransferase by a nicotinamide adenine dinucleotide-linked dehydrogenase for the utilization of mannitol.
    J Bacteriol. 1967 Feb;93(2):642-8 PMID: 4289962
  15. Membrane-bound adenosine triphosphatase of Escherichia coli. I. Partial purification and properties.
    J Biochem. 1972 Mar;71(3):387-99 PMID: 4338270
  16. Coupling of energy to active transport of amino acids in Escherichia coli.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2663-7 PMID: 4341704
  17. The membrane ATPase of Escherichia coli. I. Release into solution, allotopic properties and reconstitution of membrane-bound ATPase.
    Biochim Biophys Acta. 1973 May 30;305(2):249-59 PMID: 4354872
  18. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.128.1.248-256.1976
Published
1976-10-00
Pages
248-56
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC232850
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.128.1.248-256.1976 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2