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PMID: 3127204 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structural and functional relationships between prokaryotic and eukaryotic DNA polymerases.

The EMBO journal ·Vol. 6 ·No. 13 ·1987-12-20 ·Pages 4219-25

Bernad A, Zaballos A, Salas M, Blanco L

Abstract

The Bacillus subtilis phage luminal diameter 29 DNA polymerase, involved in protein-primed viral DNA replication, was inhibited by phosphonoacetic acid (PAA), a known inhibitor of alpha-like DNA polymerases, by decreasing the rate of elongation. Three highly conserved regions of amino acid homology, found in several viral alpha-like DNA polymerases and in the luminal diameter 29 DNA polymerase, one of them proposed to be the PAA binding site, were also found in the T4 DNA polymerase. This prokaryotic enzyme was highly sensitive to the drugs aphidicolin and the nucleotide analogues butylanilino dATP (BuAdATP) and butylphenyl dGTP (BuPdGTP), known to be specific inhibitors of eukaryotic alpha-like DNA polymerases. Two potential DNA polymerases from the linear plasmid pGKL1 from yeast and the S1 mitochondrial DNA from maize have been identified, based on the fact that they contain the three conserved regions of amino acid homology. Comparison of DNA polymerases from prokaryotic and eukaryotic origin showed extensive amino acid homology in addition to highly conserved domains. These findings reflect evolutionary relationships between hypothetically unrelated DNA polymerases.

MeSH Terms
Amino Acid Sequence Aphidicolin Bacillus subtilis/enzymology Bacteriophages/enzymology Biological Evolution DNA Replication DNA-Directed DNA Polymerase/genetics,metabolism Diterpenes/pharmacology Escherichia coli/enzymology Kinetics Mitochondria/enzymology Molecular Sequence Data Plants/enzymology Species Specificity T-Phages/enzymology Viruses/enzymology
Chemicals
Diterpenes Aphidicolin DNA-Directed DNA Polymerase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bernad A
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
Zaballos A
Salas M
Blanco L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-12-20
Pages
4219-25
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553907
Subset
IM
Grants
NIGMS NIH HHS · 2 RO1 GM27242-08 · United States
External Links
PubMed source PMC Full Text View journal details
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