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PMID: 3023690 Published · ppublish English Journal Article

Sequence and transcription analysis of the human cytomegalovirus DNA polymerase gene.

Journal of virology ·Vol. 61 ·No. 1 ·1987-01-00 ·Pages 125-33

Kouzarides T, Bankier AT, Satchwell SC, Weston K, Tomlinson P, Barrell BG

Abstract

DNA sequence analysis has revealed that the gene coding for the human cytomegalovirus (HCMV) DNA polymerase is present within the long unique region of the virus genome. Identification is based on extensive amino acid homology between the predicted HCMV open reading frame HFLF2 and the DNA polymerase of herpes simplex virus type 1. We present here a 5280-base-pair DNA sequence containing the HCMV pol gene, along with the analysis of transcripts encoded within this region. Since HCMV pol also shows homology to the predicted Epstein-Barr virus pol, we were able to analyze the extent of homology between the DNA polymerases of three distantly related herpesviruses, HCMV, Epstein-Barr virus, and herpes simplex virus. The comparison shows that these DNA polymerases exhibit considerable amino acid homology and highlights a number of highly conserved regions; two such regions show homology to sequences within the adenovirus type 2 DNA polymerase. The HCMV pol gene is flanked by open reading frames with homology to those of other herpesviruses; upstream, there is a reading frame homologous to the glycoprotein B gene of herpes simplex virus type 1 and Epstein-Barr virus, and downstream there is a reading frame homologous to BFLF2 of Epstein-Barr virus.

MeSH Terms
Amino Acid Sequence Base Sequence Cytomegalovirus/enzymology,genetics DNA Restriction Enzymes DNA-Directed DNA Polymerase/genetics Genes Genes, Viral Humans Nucleic Acid Hybridization Transcription, Genetic
Chemicals
DNA-Directed DNA Polymerase DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kouzarides T
Bankier A T
Satchwell S C
Weston K
Tomlinson P
Barrell B G
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.1.125-133.1987
Published
1987-01-00
Pages
125-33
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255219
Subset
IM
Databases
GENBANK
M14709
External Links
PubMed source DOI: 10.1128/JVI.61.1.125-133.1987 PMC Full Text View journal details
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