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PMID: 2999714 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

DNA sequence of the region in the genome of herpes simplex virus type 1 containing the genes for DNA polymerase and the major DNA binding protein.

Nucleic acids research ·Vol. 13 ·No. 22 ·1985-11-25 ·Pages 8143-63

Quinn JP, McGeoch DJ

Abstract

In the long unique region of the genome of herpes simplex virus type 1 (HSV-1), the genes for DNA polymerase and the major DNA binding protein are arranged in a head to head manner, with an origin of DNA replication (termed OriL) located between them. This paper reports an 8400 base pair DNA sequence containing both genes and the origin, obtained mostly by M13/dideoxy analysis of plasmid cloned fragments. Amino acid sequences of the two proteins were deduced. Homologues of both genes were detected in the genome sequence of the distantly related Epstein-Barr virus (EBV). Arrangement of these HSV-1 and EBV genes differs in genome location and in relative orientation. A part of HSV-1 DNA polymerase was found to be similar to a sequence in adenovirus 2 DNA polymerase, but the significance of this is unclear. Since a DNA sequence in the locality of OriL deletes on plasmid cloning, this region was analysed using virus DNA. A palindrome with 72-residue arms was found, which shows great similarity to the better characterized origin, OriS.

MeSH Terms
Adenoviridae/genetics Amino Acid Sequence Base Sequence DNA Replication DNA, Recombinant/analysis DNA-Binding Proteins/genetics DNA-Directed DNA Polymerase/genetics Herpesvirus 4, Human/genetics Sequence Homology, Nucleic Acid Simplexvirus/genetics Viral Proteins/genetics
Chemicals
DNA, Recombinant DNA-Binding Proteins Viral Proteins DNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Quinn J P
McGeoch D J
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39 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.22.8143
Published
1985-11-25
Pages
8143-63
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322116
Subset
IM
Databases
GENBANK
M12356
External Links
PubMed source DOI: 10.1093/nar/13.22.8143 PMC Full Text View journal details
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