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PMID: 3121303 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Organization of the murine immunoglobulin VH complex in the inbred strains.

The EMBO journal ·Vol. 6 ·No. 10 ·1987-10-00 ·Pages 2931-7

Rathbun GA, Capra JD, Tucker PW

Abstract

Deletion mapping analyses have been employed to order the heavy chain variable region (VH) gene families in three inbred murine strains. These nine VH gene families have been positioned with respect to the J558 and 3660 VH families in A/J (Ighe) as follows: 3609-J558-(J606,VGAM3-8,S107)-3660-(X24,Q52,7183 )-DH. Maps generated with respect to J558 in the BALB/c (Igha) and C57BL/6 (Ighb) strains are consistent with these results. The organization of the VH complex produced by deletion mapping is quite different from the accepted map generated by other methods, particularly in that J558 is more DH distal and 3660 is more DH proximal than previously thought. The order presented here is compatible with VH rearrangement frequencies suggesting preferential utilization of DH-proximal VH gene segments. Our data also indicate that interspersion of some VH family members may be a common feature of the murine VH complex since the 3609 VH family is interdigitated in the three strains and a Q52 VH gene segment is interspersed in C57BL/6.

MeSH Terms
Animals B-Lymphocytes/immunology Cell Line Chromosome Deletion Genes, Immunoglobulin Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Liver/immunology Mice Mice, Inbred A Mice, Inbred BALB C Mice, Inbred C57BL Nucleic Acid Hybridization Species Specificity
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin Variable Region
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rathbun G A
Department of Microbiology, University of Texas Health Science Center, Dallas 75235.
Capra J D
Tucker P W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-10-00
Pages
2931-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553728
Subset
IM
Grants
NIAID NIH HHS · AI-18499 · United States
NIGMS NIH HHS · GM 31689 · United States
External Links
PubMed source PMC Full Text View journal details
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