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PMID: 6806821 Published · ppublish English Journal Article

Simultaneous expression of immunoglobulin mu and delta heavy chains by a cloned B-cell lymphoma: a single copy of the VH gene is shared by two adjacent CH genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 9 ·1982-05-00 ·Pages 2996-3000

Knapp MR, Liu CP, Newell N, Ward RB, Tucker PW, Strober S, Blattner F

Abstract

The cloned murine B-cell lymphoma line (BCL1) that expresses surface IgM and IgD is considered to be a model for the immunoglobulin gene expression of the mature virgin B cell. Of particular interest is the mechanism by which a single VH gene is shared by two CH genes. We examined the organization of the immunoglobulin heavy chain genes in BCL1 DNA. A single arrangement of CH genes was found with the expressed VHDJH gene complex just 5' to the Cmu gene. The complete DNA sequence of the VH gene was determined. No rearrangement occurred in the intervening DNA between the JH and C mu genes or between the C mu and C delta genes. We conclude that dual expression of mu and delta heavy chains using a single VH gene is accomplished by alternate processing of a primary transcript that encompasses the the VHDJH complex and both CH genes.

MeSH Terms
Animals B-Lymphocytes/immunology Binding Sites, Antibody/genetics Cell Line DNA, Neoplasm/genetics Gene Expression Regulation Genes Immunoglobulin Heavy Chains/genetics Immunoglobulin Idiotypes/genetics Immunoglobulin Variable Region/genetics Immunoglobulin delta-Chains/genetics Immunoglobulin mu-Chains/genetics Lymphoma/genetics,immunology Mice Receptors, Antigen, B-Cell/genetics Recombination, Genetic
Chemicals
DNA, Neoplasm Immunoglobulin Heavy Chains Immunoglobulin Idiotypes Immunoglobulin Variable Region Immunoglobulin delta-Chains Immunoglobulin mu-Chains Receptors, Antigen, B-Cell
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Knapp M R
Liu C P
Newell N
Ward R B
Tucker P W
Strober S
Blattner F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.9.2996
Published
1982-05-00
Pages
2996-3000
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346335
Subset
IM
Databases
GENBANK
J00494
External Links
PubMed source DOI: 10.1073/pnas.79.9.2996 PMC Full Text View journal details
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