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PMID: 116236 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloned pairs of variable region genes for immunoglobulin heavy chains isolated from a clone library of the entire mouse genome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 9 ·1979-09-00 ·Pages 4627-31

Kemp DJ, Cory S, Adams JM

Abstract

To investigate the organization of immunoglobulin genes, we have constructed a clone library containing 10(6) randomly generated fragments of mouse embryo DNA, corresponding to eight equivalents of the genome. The cloning method involved methylation of embryo DNA at EcoRI recognition sites, partial digestion by EcoRI* endonclease activity, and direct ligation of the resulting large fragments to the lambda phage vector Charon 4A. The library was searched for sequences homologous to a cloned complementary DNA copy of a mu heavy chain mRNA. Nine clones bearing variable heavy chain (VH) sequences were isolated, representing at least eight distinct VH genes. Thus, multiple related VH genes are available in the genome to contribute to immunoglobulin diversity. Each of the two clones carries a pair of VH genes, one pair separated by 15 +/- 1 kilobase pairs of mouse DNA and the other by 14 +/- 2 kilobase pairs. This indicates that related VH genes are clustered and may occur in a tandem array having a repeating unit of 14--16 kilobase pairs. The large spacer sequences between VH genes cannot, however, be highly conserved.

MeSH Terms
Animals Antibody Diversity Binding Sites, Antibody/genetics Chromosome Mapping DNA, Recombinant Embryo, Mammalian/immunology Genetic Linkage Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Immunoglobulin mu-Chains/genetics Mice RNA, Messenger/genetics
Chemicals
DNA, Recombinant Immunoglobulin Heavy Chains Immunoglobulin Variable Region Immunoglobulin mu-Chains RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kemp D J
Cory S
Adams J M
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.9.4627
Published
1979-09-00
Pages
4627-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411632
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.9.4627 PMC Full Text View journal details
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