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PMID: 3112524 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Plasmid maintenance in Bacillus subtilis recombination-deficient mutants.

Molecular & general genetics : MGG ·Vol. 208 ·No. 1-2 ·1987-06-00 ·Pages 349-52

Alonso JC, Viret JF, Tailor RH

Abstract

An isogenic set of 11 recombination-deficient mutant strains of Bacillus subtilis has been constructed. Whereas plasmid pUB110 is stably maintained in such Rec- cells, the high copy number plasmid pC194 is unstable. Instability in Rec- strains could be mostly attributed to the deleterious effect of the presence of the plasmid on the Rec- cells' growth capability. In part, instability of pC194 derivatives could also be correlated with the presence of an unusually high amount of multimeric DNA molecules.

MeSH Terms
Bacillus subtilis/genetics Gene Expression Regulation Mutation Plasmids Recombination, Genetic
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Alonso J C
Viret J F
Tailor R H
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27 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330464
Published
1987-06-00
Pages
349-52
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330464
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