Home LiteratureArticle Details
PMID: 3085097 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Single-stranded plasmid DNA in Bacillus subtilis and Staphylococcus aureus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 8 ·1986-04-00 ·Pages 2541-5

te Riele H, Michel B, Ehrlich SD

Abstract

Plasmid pC194 was found to exist in a double-stranded and a single-stranded DNA form in Bacillus subtilis and Staphylococcus aureus. This single-stranded DNA was found as a circular molecule of the same size as the parental monomer and corresponded to only one of the two DNA strands. It represented one-third of plasmid copies. Single- and double-stranded DNA copies in similar proportions to the above were detected for five other S. aureus plasmids (pC221, pC223, pE194, pT127, and pT181) and one B. subtilis plasmid (pHV416). S. aureus plasmid pUB110 and Bacillus cereus plasmid pBC16 were, in contrast, predominantly double-stranded.

MeSH Terms
Bacillus subtilis/genetics DNA, Bacterial/genetics DNA, Circular/genetics DNA, Single-Stranded/genetics Microscopy, Electron Plasmids Staphylococcus aureus/genetics
Chemicals
DNA, Bacterial DNA, Circular DNA, Single-Stranded
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
te Riele H
Michel B
Ehrlich S D
References (31)
31 references, click to expand
  1. Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilis.
    J Bacteriol. 1978 Feb;133(2):897-903 PMID: 415051
  2. Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1977 Apr;74(4):1680-2 PMID: 404641
  3. Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194.
    J Bacteriol. 1979 Jan;137(1):635-43 PMID: 104975
  4. Incompatibility and molecular relationships between small Staphylococcal plasmids carrying the same resistance marker.
    Plasmid. 1978 Sep;1(4):468-79 PMID: 748954
  5. Insertion mutant of bacteriophage f1 sensitive to EcoRI.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2699-702 PMID: 379863
  6. In vitro genetic labeling of Bacillus subtilis cryptic plasmid pHV400.
    Plasmid. 1979 Jan;2(1):48-58 PMID: 112601
  7. DNA replication of bacteriophage f1 in vivo.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:389-99 PMID: 225108
  8. Replication of the colicin E1 plasmid in extracts of Escherichia coli: uncoupling of leading strand from lagging strand synthesis.
    Mol Gen Genet. 1979;177(1):113-20 PMID: 231724
  9. Replication of plasmids from Staphylococcus aureus in Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7333-7 PMID: 7012836
  10. Use of plasmids from Staphylococcus aureus for cloning of DNA in Bacillus subtilis.
    Curr Top Microbiol Immunol. 1982;96:19-29 PMID: 6276091
  11. Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibodies.
    J Bacteriol. 1982 May;150(2):804-14 PMID: 6279574
  12. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance.
    J Bacteriol. 1982 May;150(2):815-25 PMID: 6950931
  13. Closely related plasmids from Staphylococcus aureus and soil bacilli.
    Plasmid. 1982 Mar;7(2):152-62 PMID: 7079389
  14. Plasmids from Staphylococcus aureus replicate in yeast Saccharomyces cerevisiae.
    Nature. 1982 Jul 29;298(5873):488-90 PMID: 6283393
  15. Functional origin of replication of pT181 plasmid DNA is contained within a 168-base-pair segment.
    Proc Natl Acad Sci U S A. 1982 Aug;79(15):4580-4 PMID: 6956881
  16. The filamentous phage (Ff) as vectors for recombinant DNA--a review.
    Gene. 1982 Jul-Aug;19(1):1-10 PMID: 6292041
  17. Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid from Staphylococcus aureus.
    Plasmid. 1983 Nov;10(3):251-9 PMID: 6657777
  18. Maintenance of multicopy plasmid Clo DF13 in E. coli cells: evidence for site-specific recombination at parB.
    Cell. 1984 Jan;36(1):203-9 PMID: 6319008
  19. Multimerization of high copy number plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability.
    Cell. 1984 Apr;36(4):1097-103 PMID: 6323019
  20. Replication functions of pC194 are necessary for efficient plasmid transduction by M13 phage.
    EMBO J. 1984 Jan;3(1):81-6 PMID: 6323171
  21. Control of pT181 replication I. The pT181 copy control function acts by inhibiting the synthesis of a replication protein.
    EMBO J. 1984 Oct;3(10):2399-405 PMID: 6499834
  22. Recombination between repeated DNA sequences occurs more often in plasmids than in the chromosome of Bacillus subtilis.
    Mol Gen Genet. 1984;197(1):46-54 PMID: 6439976
  23. Biology of bacterial deoxyribonucleic acid topoisomerases.
    Microbiol Rev. 1984 Dec;48(4):273-89 PMID: 6097803
  24. The use of synthetic oligonucleotides with universal templates for rapid DNA sequencing: results with staphylococcal replicon pC221.
    EMBO J. 1985 Feb;4(2):561-8 PMID: 3860383
  25. Comparative sequence and functional analysis of pT181 and pC221, cognate plasmid replicons from Staphylococcus aureus.
    Mol Gen Genet. 1985;199(3):452-64 PMID: 2993795
  26. Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage lambda by electron microscopy.
    Science. 1969 Mar 21;163(3873):1343-8 PMID: 5765116
  27. Models of genetic recombination.
    Annu Rev Microbiol. 1974;28(0):445-68 PMID: 4611334
  28. A general model for genetic recombination.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):358-61 PMID: 1054510
  29. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  30. Uniform nomenclature for bacterial plasmids: a proposal.
    Bacteriol Rev. 1976 Mar;40(1):168-89 PMID: 1267736
  31. Analysis of the phiX DNA replication cycle by electron microscopy.
    Proc Natl Acad Sci U S A. 1978 Feb;75(2):605-9 PMID: 273222
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.8.2541
Published
1986-04-00
Pages
2541-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323334
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.8.2541 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2