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PMID: 7012836 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Replication of plasmids from Staphylococcus aureus in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 12 ·1980-12-00 ·Pages 7333-7

Goze A, Ehrlich SD

Abstract

Plasmid pBR322 derives from plasmid ColE1 and does not replicate in Escherichia coli strains lacking DNA polymerase I. Hybrids between pBR322 and a plasmid isolated from Staphylococcus aureus, pC194, replicate in such E. coli strains, provided that the pC194 replication region is intact. Inactivation of the pBR322 replication region does not interfere with the replication of hybrids in E. coli. Hybrids between pBR322 and two other plasmids from S. aureus, pT127 and pUB112, and replicate at the restrictive temperature in E. coli having thermosensitive DNA polymerase I. Similar hybrids involving pC221 and pHV400, plasmids from S. aureus and Bacillus subtilis, respectively, do not replicate under such conditions. These results show that some plasmids from a Gram-positive bacterium, S. aureus, can replicate in a Gram-negative one, E. coli.

MeSH Terms
Base Sequence DNA Replication DNA-Directed DNA Polymerase/metabolism Escherichia coli/genetics Plasmids Staphylococcus aureus/genetics
Chemicals
DNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goze A
Ehrlich S D
References (34)
34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.12.7333
Published
1980-12-00
Pages
7333-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350497
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.12.7333 PMC Full Text View journal details
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