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PMID: 3925293 Published · ppublish English Journal Article

A gene controlling segregation of the Bacillus subtilis plasmid pC194.

Molecular & general genetics : MGG ·Vol. 198 ·No. 3 ·1985-00-00 ·Pages 427-31

Alonso JC, Trautner TA

Abstract

Plasmid pC194-1, a mutant of pC194, and chimeric derivatives of pC194-1 are segregationally unstable in B. subtilis. Such instability could be enhanced by exposure of pC194-1-carrying cells to methyl methanesulfonate. pC194-1 is distinct from pC194 in the addition of two A:T base pairs within the previously defined D region of pC194. Complementation experiments between pC194-1 and other plasmids suggest that the mutation of pC194-1 interferes with the production of a diffusible gene product required for plasmid maintenance.

MeSH Terms
Bacillus subtilis/genetics Genes, Bacterial Genetic Complementation Test Methyl Methanesulfonate/pharmacology Plasmids/drug effects,radiation effects Recombination, Genetic Transformation, Bacterial Ultraviolet Rays
Chemicals
Methyl Methanesulfonate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Alonso J C
Trautner T A
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25 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00332934
Published
1985-00-00
Pages
427-31
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00332934
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