Cold sensitivity in the transfer of a plasmid with a deletion hot spot into recombination deficient B. subtilis cells.

Molecular & general genetics : MGG ·Vol. 198 ·No. 3 ·1985-00-00 ·Pages 437-40

Alonso JC, Trautner TA

Abstract

Various recombination-deficient mutants of B. subtilis, which are readily transformable by plasmid DNA at 42 degrees C cannot be transformed at 30 degrees C with chimeric plasmid derivatives that contain the deletion hot spot defined previously (Alonso and Trautner 1985a, b). Such interference was also observed in protoplast transformation and SPP1 transduction.

MeSH Terms

Bacillus subtilis/genetics,ultrastructure Chromosome Deletion Chromosomes, Bacterial/ultrastructure Cold Temperature Nucleic Acid Conformation Plasmids Recombination, Genetic Replicon Transformation, Bacterial

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Alonso J C

Trautner T A

References (11)

11 references, click to expand

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Generation of deletions through a cis-acting mutation in plasmid pC194.
Mol Gen Genet. 1985;198(3):432-6 PMID: 3925294
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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00332936

Published

1985-00-00

Pages

437-40

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00332936

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Cold sensitivity in the transfer of a plasmid with a deletion hot spot into recombination deficient B. subtilis cells.

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