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PMID: 3108882 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Protein-nucleotide contacts in the immunoglobulin heavy-chain promoter region.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 11 ·1987-06-00 ·Pages 3851-5

Landolfi NF, Capra JD, Tucker PW

Abstract

Immunoglobulin heavy-chain variable-region genes contain the octanucleotide ATGCAAAT upstream from the site of transcription initiation. The complement of this sequence, in the reverse orientation, is found at an identical location in light-chain variable-region genes. This sequence element is thought to be involved in the lymphoid-specific expression of immunoglobulin genes. Analysis of nuclear extracts from both lymphoid and nonlymphoid cells in a gel migration inhibition assay, using an immunoglobulin promoter region fragment containing the octamer, reveals multiple migration-retarded species that represent specific DNA-protein complexes. The number and relative level of these complexes vary with cell type; some complexes are detected with all extracts, whereas one complex is lymphoid-specific and may represent an interaction involved in the lymphoid-restricted expression of immunoglobulin genes. Mitogenic stimulation of a B-lymphoid line can increase the level of the protein responsible for this lymphoid-specific complex. Analysis of the complexes detected in the gel migration inhibition assay by DNase I protection ("footprinting") has revealed that all of these DNA-protein complexes involve contact of the protein with the nucleotides of the octamer. One complex, present in both lymphoid and nonlymphoid cells, displays an additional DNA-protein contact adjacent to the octamer. Our results also indicate that the interaction of proteins with the octameric sequence can cause a local alteration in the structure of the DNA helix.

MeSH Terms
Animals B-Lymphocytes/immunology Base Sequence Deoxyribonuclease I Genes Immunoglobulin Heavy Chains/genetics Leukemia, Experimental/immunology Lymphocyte Activation Mice Nucleic Acid Conformation Promoter Regions, Genetic Transcription, Genetic
Chemicals
Immunoglobulin Heavy Chains Deoxyribonuclease I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Landolfi N F
Capra J D
Tucker P W
References (33)
33 references, click to expand
  1. Characterization of a spontaneous murine B cell leukemia (BCL1). II. Tumor cell proliferation and IgM secretion after stimulation by LPS.
    J Immunol. 1979 Sep;123(3):1000-6 PMID: 381513
  2. Separation of DNA binding from the transcription-activating function of a eukaryotic regulatory protein.
    Science. 1986 Feb 14;231(4739):699-704 PMID: 3080805
  3. Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2650-4 PMID: 6425835
  4. Cell-type specific protein binding to the enhancer of simian virus 40 in nuclear extracts.
    Nature. 1986 Oct 9-15;323(6088):544-8 PMID: 3020434
  5. An immunoglobulin promoter displays cell-type specificity independently of the enhancer.
    Nature. 1985 May 30-Jun 5;315(6018):423-5 PMID: 3923370
  6. Structure of two unlinked Drosophila melanogaster glyceraldehyde-3-phosphate dehydrogenase genes.
    J Biol Chem. 1985 Jul 5;260(13):8220-8 PMID: 2989282
  7. Simultaneous expression of immunoglobulin mu and delta heavy chains by a cloned B-cell lymphoma: a single copy of the VH gene is shared by two adjacent CH genes.
    Proc Natl Acad Sci U S A. 1982 May;79(9):2996-3000 PMID: 6806821
  8. A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes.
    Nature. 1986 Oct 16-22;323(6089):640-3 PMID: 3095662
  9. Contribution of promoter to tissue-specific expression of the mouse immunoglobulin kappa gene.
    Science. 1985 Sep 13;229(4718):1102-4 PMID: 2994213
  10. Genomic organization and nucleotide sequence of two distinct histone gene clusters from Xenopus laevis. Identification of novel conserved upstream sequence elements.
    J Mol Biol. 1985 Oct 5;185(3):479-99 PMID: 3863963
  11. Two regulatory elements for immunoglobulin kappa light chain gene expression.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7041-5 PMID: 6438631
  12. Cell-type specificity of immunoglobulin gene expression is regulated by at least three DNA sequence elements.
    Cell. 1985 Jul;41(3):885-97 PMID: 3924411
  13. Bending of promoter DNA on binding of heat shock transcription factor.
    Nature. 1986 Oct 2-8;323(6087):459-61 PMID: 3020432
  14. Orientation-dependent transcriptional activator upstream of a human U2 snRNA gene.
    Mol Cell Biol. 1985 Jul;5(7):1560-70 PMID: 2410771
  15. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
    Nature. 1986 Jan 9-15;319(6049):154-8 PMID: 3079885
  16. Multiple sequence elements are required for maximal in vitro transcription of a human histone H2B gene.
    Mol Cell Biol. 1986 Oct;6(10):3329-40 PMID: 3025588
  17. Activated T-lymphocytes express class I molecules which are hyposialylated compared to other lymphocyte populations.
    Mol Immunol. 1986 Mar;23(3):297-309 PMID: 3487029
  18. Multiple nuclear factors interact with the immunoglobulin enhancer sequences.
    Cell. 1986 Aug 29;46(5):705-16 PMID: 3091258
  19. Transcriptional regulation of the mu-delta heavy chain locus in normal murine B lymphocytes.
    J Exp Med. 1984 Aug 1;160(2):564-83 PMID: 6206182
  20. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  21. Transcription signals in embryonic Xenopus laevis U1 RNA genes.
    EMBO J. 1985 Jun;4(6):1537-43 PMID: 2411541
  22. Correct transcription of an immunoglobulin kappa gene requires an upstream fragment containing conserved sequence elements.
    Nature. 1984 Jul 5-11;310(5972):71-4 PMID: 6330567
  23. An enhancer-like sequence within the Xenopus U2 gene promoter facilitates the formation of stable transcription complexes.
    Nature. 1985 Jul 11-17;316(6024):163-7 PMID: 2409453
  24. Nuclear factors bind to regulatory regions of the mouse kappa immunoglobulin gene.
    Nucleic Acids Res. 1986 Jun 25;14(12):4837-48 PMID: 3088544
  25. Interaction of a common factor with conserved promoter and enhancer sequences in histone H2B, immunoglobulin, and U2 small nuclear RNA (snRNA) genes.
    Proc Natl Acad Sci U S A. 1986 Sep;83(17):6382-6 PMID: 3462701
  26. The two embryonic U1 RNA genes of Xenopus laevis have both common and gene-specific transcription signals.
    EMBO J. 1985 Jun;4(6):1529-35 PMID: 2411540
  27. Cloning of a gene localized and expressed at the ecdysteroid regulated puff 74EF in salivary glands of Drosophila larvae.
    EMBO J. 1984 Feb;3(2):289-95 PMID: 16453498
  28. DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
    Nucleic Acids Res. 1978 Sep;5(9):3157-70 PMID: 212715
  29. Interaction of cell-type-specific nuclear proteins with immunoglobulin VH promoter region sequences.
    Nature. 1986 Oct 9-15;323(6088):548-51 PMID: 3093895
  30. Structure and nucleotide sequence of the heavy chain gene of HLA-DR.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3543-7 PMID: 6304715
  31. Transcription cell type specificity is conferred by an immunoglobulin VH gene promoter that includes a functional consensus sequence.
    Cell. 1985 Jun;41(2):479-87 PMID: 3921262
  32. Cell-type preference of immunoglobulin kappa and lambda gene promoters.
    EMBO J. 1985 Nov;4(11):2831-8 PMID: 2998757
  33. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.11.3851
Published
1987-06-00
Pages
3851-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304974
Subset
IM
Grants
NIAID NIH HHS · AI-18016 · United States
NIGMS NIH HHS · GM-1751 · United States
NIGMS NIH HHS · GM-31689 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.11.3851 PMC Full Text View journal details
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