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PMID: 3108855 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Pseudomonas aeruginosa infection in cystic fibrosis: nucleotide sequence and transcriptional regulation of the algD gene.

Nucleic acids research ·Vol. 15 ·No. 11 ·1987-06-11 ·Pages 4567-81

Deretic V, Gill JF, Chakrabarty AM

Abstract

Pulmonary infection by mucoid, alginate producing, Pseudomonas aeruginosa is a major complication in patients suffering from cystic fibrosis (CF). To analyze the mechanisms leading to the emergence of mucoid P. aeruginosa in CF lungs, control of the algD gene coding for GDPmannose dehydrogenase was studied. Transcriptional activation of algD was shown to be necessary for alginate production. Sequencing of algD and its promoter revealed multiple direct repeats upstream of the transcription start and throughout the promoter region. Using the algD-xy1E transcriptional fusion the algD promoter was demonstrated to be under positive control by the algR gene. This gene has previously been shown to undergo antibiotic promoted chromosomal amplification resulting in the emergence of the mucoid phenotype. These findings provide a basis for better understanding the control of mucoidy in P. aeruginosa.

MeSH Terms
Alginates/biosynthesis Amino Acid Sequence Base Sequence Carbohydrate Dehydrogenases/genetics Cystic Fibrosis/microbiology Gene Expression Regulation Genes, Bacterial Genes, Regulator Humans Nucleic Acid Conformation Promoter Regions, Genetic Pseudomonas aeruginosa/genetics Repetitive Sequences, Nucleic Acid Transcription, Genetic
Chemicals
Alginates Carbohydrate Dehydrogenases GDPmannose dehydrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Deretic V
Gill J F
Chakrabarty A M
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38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.11.4567
Published
1987-06-11
Pages
4567-81
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340880
Subset
IM
Grants
NIAID NIH HHS · AI 16790-08 · United States
Databases
GENBANK
Y00337
External Links
PubMed source DOI: 10.1093/nar/15.11.4567 PMC Full Text View journal details
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