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PMID: 3519576 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

High A + T content conserved in DNA sequences upstream of leuABCD in Escherichia coli and Salmonella typhimurium.

Journal of bacteriology ·Vol. 166 ·No. 3 ·1986-06-00 ·Pages 1113-7

Haughn GW, Wessler SR, Gemmill RM, Calvo JM

Abstract

The nucleotide sequence of over 800 base pairs of DNA upstream of leuP was determined for Escherichia coli and Salmonella typhimurium. In both of these enteric bacteria, approximately 500 base pairs of A + T-rich sequences separates leuP from an upstream open reading frame. Although these A + T-rich sequences share little homology, the distribution of A + T base pairs within the region is strikingly conserved. Deletion of the A + T-rich sequences upstream of the E. coli leu operon does not markedly affect the strength of the leu promoter in vivo.

MeSH Terms

Adenine/analysis Base Sequence DNA, Bacterial/analysis Escherichia coli/genetics Galactokinase/metabolism Plasmids Salmonella typhimurium/genetics Thymine/analysis

Chemicals

DNA, Bacterial Galactokinase Adenine Thymine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Haughn G W

Wessler S R

Gemmill R M

Calvo J M

References (28)

28 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.166.3.1113-1117.1986

Published

1986-06-00

Pages

1113-7

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC215239

Subset

Grants

NIAID NIH HHS · AI14340 · United States

Databases

GENBANK

M12891, M12892

External Links

PubMed source DOI: 10.1128/jb.166.3.1113-1117.1986 PMC Full Text View journal details

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High A + T content conserved in DNA sequences upstream of leuABCD in Escherichia coli and Salmonella typhimurium.

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