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PMID: 6308579 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Molecular structure of ilvIH and its evolutionary relationship to ilvG in Escherichia coli K12.

Nucleic acids research ·Vol. 11 ·No. 15 ·1983-08-11 ·Pages 5299-313

Squires CH, De Felice M, Devereux J, Calvo JM

Abstract

ilvIH of Escherichia coli K12 codes for a valine-sensitive acetohydroxy acid synthase (AHASIII). The DNA sequence of ilvIH was determined. Open reading frames and appropriate translation signals exist for two polypeptides, one containing 565 amino acids (ilvI polypeptide) and the other 160 amino acids (ilvH polypeptide). A graphic matrix analysis shows three clearcut regions of homology between ilvI and ilvG (codes for AHASII). Within these three regions of homology, 50-60% of the amino acid sequences of AHASII and AHASIII are conserved. Inspection of the region between ilvG and ilvE (the K region) revealed that it can potentially code for an 86 amino acid polypeptide. A computer analysis shows small but significant homology between the predicted amino acid sequences of the N-terminal half of the ilvH polypeptide and the putative region K polypeptide. We conclude that ilvIH and ilvG-region K evolved from a common ancestor.

MeSH Terms

Acetolactate Synthase/genetics Amino Acid Sequence Base Sequence Biological Evolution DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Bacterial Isoenzymes/genetics Molecular Sequence Data Oxo-Acid-Lyases/genetics Structure-Activity Relationship Valine/pharmacology

Chemicals

Isoenzymes Acetolactate Synthase DNA Restriction Enzymes Oxo-Acid-Lyases Valine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Squires C H

De Felice M

Devereux J

Calvo J M

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26 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/11.15.5299

Published

1983-08-11

Pages

5299-313

Language

English

Region

England

NLM ID

0411011

PMCID

PMC326267

Subset

Databases

GENBANK

X01609

External Links

PubMed source DOI: 10.1093/nar/11.15.5299 PMC Full Text View journal details

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Molecular structure of ilvIH and its evolutionary relationship to ilvG in Escherichia coli K12.

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