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PMID: 3104910 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Functional analysis of a palindromic sequence required for normal replication of several staphylococcal plasmids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 8 ·1987-04-00 ·Pages 2165-9

Gruss AD, Ross HF, Novick RP

Abstract

Most small multicopy antibiotic-resistance plasmids of Staphylococcus aureus contain a major axis of hyphenated dyad symmetry (palA) that is required for normal replication and stability, although located outside of the minimal replicon. Rearrangements affecting palA cause plasmid instability, a marked reduction in copy number, and the accumulation of large quantities of strand-specific circular single-stranded plasmid DNA. In view of the recent observation that pT181 initiates replication by a nick and 3'-extension mechanism (S. Khan, personal communication), it is suggested that these plasmids replicate by an asymmetric rolling-circle mechanism in which the displaced plus strand remains single stranded until palA is exposed, forming a hairpin that serves as the lagging strand origin.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacillus subtilis/genetics Base Sequence Chromosome Deletion DNA Replication Drug Resistance, Microbial R Factors Staphylococcus aureus/drug effects,genetics
Chemicals
Anti-Bacterial Agents
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gruss A D
Ross H F
Novick R P
References (32)
32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.8.2165
Published
1987-04-00
Pages
2165-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304609
Subset
IM
Grants
NIGMS NIH HHS · GM14372 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.8.2165 PMC Full Text View journal details
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