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PMID: 3087948 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Sequence and properties of pIM13, a macrolide-lincosamide-streptogramin B resistance plasmid from Bacillus subtilis.

Journal of bacteriology ·Vol. 167 ·No. 1 ·1986-07-00 ·Pages 138-47

Monod M, Denoya C, Dubnau D

Abstract

We initiated a study of pIM13, a multicopy, macrolide-lincosamide-streptogramin B (MLS) plasmid first isolated from a strain of Bacillus subtilis and described by Mahler and Halvorson (J. Gen. Microbiol. 120:259-263, 1980). The copy number of this plasmid was about 200 in B. subtilis and 30 in Staphylococcus aureus. The MLS resistance determinant of pIM13 was shown to be highly homologous to ermC, an inducible element on the S. aureus plasmid pE194. The product of the pIM13 determinant was similar in size to that of ermC and immunologically cross-reactive with it. The MLS resistance of pIM13 was expressed constitutively. The complete base sequence of pIM13 is presented. The plasmid consisted of 2,246 base pairs and contained two open reading frames that specified products identified in minicell extracts. One was a protein of 16,000 molecular weight, possibly required for replication. The second was the 29,000-molecular-weight MLS resistance methylase. The regulatory region responsible for ermC inducibility was missing from pIM13, explaining its constitutivity. The remainder of the pIM13 MLS determinant was nearly identical to ermC. The ends of the region of homology between pIM13 and pE194 were associated with hyphenated dyad symmetries. A segment partially homologous to one of these termini on pIM13 and also associated with a dyad was found in pUB110 near the end of a region of homology between that plasmid and pBC16. The entire sequence of pIM13 was highly homologous to that of pE5, an inducible MLS resistance plasmid from S. aureus that differs from pIM13 in copy control.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacillus subtilis/drug effects,genetics,metabolism Base Sequence DNA Replication DNA, Bacterial Electrophoresis, Agar Gel Lincosamides Macrolides Methyltransferases/metabolism R Factors Staphylococcus aureus/drug effects,genetics Virginiamycin/pharmacology
Chemicals
Anti-Bacterial Agents DNA, Bacterial Lincosamides Macrolides Virginiamycin Methyltransferases rRNA (adenosine-O-2'-)methyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Monod M
Denoya C
Dubnau D
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47 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.167.1.138-147.1986
Published
1986-07-00
Pages
138-47
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212852
Subset
IM
Grants
NIAID NIH HHS · AI10311 · United States
NIAID NIH HHS · AI17472 · United States
Databases
GENBANK
M13761
External Links
PubMed source DOI: 10.1128/jb.167.1.138-147.1986 PMC Full Text View journal details
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