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PMID: 105246 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of plasmid transformation in Bacillus subtilis: kinetic properties and the effect of DNA conformation.

Molecular & general genetics : MGG ·Vol. 167 ·No. 3 ·1979-01-02 ·Pages 251-8

Contente S, Dubnau D

Abstract

Transformation of competent cells of Bacillus subtilis with antibiotic resistance plasmid DNA has shown that (a) competence for plasmid and chromosomal DNA develops with similar kinetics; (b) DNA linearized with a variety of restriction endonucleases does not transform; (c) CCC plasmid DNA is inactivated for transformation by a single nick; (d) T4 ligase restores transforming activity to both nicked and linearized DNA; (E) CCC relaxed DNA is fully active in transformation; (f) the DNA concentration-dependence of plasmid transformation is first order; and (g) plasmid transformation proceeds with a low efficiency, requiring the uptake of 10(3) to 10(4) DNA molecules per transformant. Based on this information, a model for the processing of chromosomal, plasmid and transfecting DNA is proposed.

MeSH Terms
Bacillus subtilis/genetics DNA, Bacterial/genetics Kinetics Models, Biological Nucleic Acid Conformation Plasmids Recombination, Genetic Transfection Transformation, Bacterial
Chemicals
DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Contente S
Dubnau D
References (22)
22 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267416
Published
1979-01-02
Pages
251-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267416
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