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PMID: 3097517 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequence of the notch locus of Drosophila melanogaster: relationship of the encoded protein to mammalian clotting and growth factors.

Molecular and cellular biology ·Vol. 6 ·No. 9 ·1986-09-00 ·Pages 3094-108

Kidd S, Kelley MR, Young MW

Abstract

The Notch locus is essential for proper differentiation of the ectoderm in Drosophila melanogaster. Notch corresponds to a 37-kilobase transcription unit that codes for a major 10.4-kilobase polyadenylated RNA. The DNA sequence of this transcription unit is presented, except for portions of the two largest intervening sequences. DNA sequences also were obtained from three Notch cDNA clones, allowing the 5' and 3' ends of the gene to be mapped, and the structures and locations of nine RNA coding regions to be determined. The major Notch transcript encodes a protein of 2,703 amino acids. The protein is probably associated with cell surfaces and carries an extracellular domain composed of 36 cysteine-rich repeating units, each of about 38 amino acids. The gene appears to have evolved by repeated tandem duplications of the DNA coding for the 38-amino-acid-long protein segments, followed by insertion of intervening sequences. These repeating protein segments are quite homologous to portions of mammalian clotting factors IX and X and to the product of the Caenorhabditis elegans developmental gene lin-12. They are also similar to mammalian growth hormones, typified by epidermal growth factor.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/analysis Drosophila melanogaster/genetics Epidermal Growth Factor/genetics Factor IX/genetics Factor X/genetics Genes Genes, Developmental Growth Substances/genetics Membrane Proteins/genetics Sequence Homology, Nucleic Acid Software Transcription, Genetic
Chemicals
Growth Substances Membrane Proteins Epidermal Growth Factor Factor IX Factor X DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kidd S
Kelley M R
Young M W
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54 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.9.3094-3108.1986
Published
1986-09-00
Pages
3094-108
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367044
Subset
IM
Databases
GENBANK
K03507, K03508, M13689
External Links
PubMed source DOI: 10.1128/mcb.6.9.3094-3108.1986 PMC Full Text View journal details
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