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PMID: 6091100 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning and sequencing of liver cDNA coding for bovine protein C.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 18 ·1984-09-00 ·Pages 5653-6

Long GL, Belagaje RM, MacGillivray RT

Abstract

cDNA coding for protein C has been cloned from a bovine liver library in plasmid vector pBR322 and its sequence has been determined. Two overlapping clones code for the entire light and heavy chains of the mature protein, as well as a previously unreported connecting dipeptide (Lys-Arg) and a 39-amino acid leader peptide region. Identification and characterization of the clones establishes the liver as a site of protein C biosynthesis. A contiguous coding region reveals that a one-chain precursor protein is made that upon limited proteolysis yields both the mature light and heavy chains. The codon for aspartic acid is found at light chain amino acid position 71, showing that the beta-hydroxyaspartic acid that exists in this position of the mature protein is the result of post-translational modification of an aspartic acid residue. Amino acid sequence homology in the amino-terminal region of the light chain with other vitamin K-dependent coagulation factors is continued into the leader peptide region.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blood Coagulation Factors/genetics Cattle Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Factor IX/genetics Factor X/genetics Glycoproteins/genetics Humans Liver/enzymology Nucleic Acid Hybridization Oligodeoxyribonucleotides Plasmids Protein C Prothrombin/genetics RNA, Messenger/genetics
Chemicals
Blood Coagulation Factors Glycoproteins Oligodeoxyribonucleotides Protein C RNA, Messenger Prothrombin Factor IX Factor X DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Long G L
Belagaje R M
MacGillivray R T
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.18.5653
Published
1984-09-00
Pages
5653-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391768
Subset
IM
Databases
GENBANK
K02435
External Links
PubMed source DOI: 10.1073/pnas.81.18.5653 PMC Full Text View journal details
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