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PMID: 6330671 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Blood coagulation factor X mRNA encodes a single polypeptide chain containing a prepro leader sequence.

Nucleic acids research ·Vol. 12 ·No. 11 ·1984-06-11 ·Pages 4481-92

Fung MR, Campbell RM, MacGillivray RT

Abstract

Thirty thousand colonies of a bovine liver cDNA library were screened with a mixture of synthetic oligodeoxyribonucleotides coding for bovine factor X. Five positive colonies were identified, and plasmid DNA was isolated. Cleavage with restriction endonucleases showed that these plasmids (designated pBX1 -5) contained inserts of 1530bp , 770bp , 700bp , 1100bp and 930bp . DNA sequence analysis of the plasmid with the largest insert ( pBX1 ) confirmed that bovine factor X cDNAs had been cloned. The cDNA sequence predicts that factor X is synthesized as a single chain precursor in which the light and heavy chains of plasma factor X are linked by the dipeptide Arg-Arg. The cDNA sequence also predicts that factor X is synthesized with a prepro leader peptide. We propose that at least five specific proteolytic events occur during the conversion of prepro -factor X to plasma factor Xa.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cattle Cloning, Molecular DNA/analysis DNA Restriction Enzymes Factor IX/genetics Factor X/genetics Genes Humans Oligodeoxyribonucleotides/genetics Protein Precursors/genetics Prothrombin/genetics RNA, Messenger/genetics
Chemicals
Oligodeoxyribonucleotides Protein Precursors RNA, Messenger Prothrombin Factor IX Factor X DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fung M R
Campbell R M
MacGillivray R T
References (23)
23 references, click to expand
  1. An examination of conditions for the cleavage of polypeptide chains with cyanogen bromide: application to catalase.
    Arch Biochem Biophys. 1969 Mar;130(1):551-6 PMID: 5778667
  2. A form of bovine factor X with a single polypeptide chain.
    Nat New Biol. 1973 Mar 21;242(116):90-2 PMID: 4512238
  3. The mechanism of activation of bovine factor X (Stuart factor) by intrinsic and extrinsic pathways.
    Biochemistry. 1974 Dec 17;13(26):5290-9 PMID: 4474002
  4. Activation of human prothrombin by highly purified human factors V and X-a in presence of human antithrombin.
    J Biol Chem. 1975 Mar 10;250(5):1607-17 PMID: 1112820
  5. Bovine factor X1 (Stuart factor): amino-acid sequence of heavey chain.
    Proc Natl Acad Sci U S A. 1975 Aug;72(8):3082-6 PMID: 1059093
  6. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  7. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  8. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
  9. Conversion of rat pre-proalbumin to proalbumin in vitro by ascites membranes. Demonstration by NH2-TERMINAL SEQUENCE ANALYSIS.
    J Biol Chem. 1978 Sep 10;253(17):6270-4 PMID: 681351
  10. Precursor of egg white ovomucoid. Amino acid sequence of an NH2-terminal extension.
    J Biol Chem. 1978 Dec 25;253(24):9018-23 PMID: 721826
  11. Amino acid sequence of the light chain of bovine factor X1 (Stuart factor).
    Biochemistry. 1980 Feb 19;19(4):659-67 PMID: 6766735
  12. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  13. 5'-32P labeling of RNA and DNA restriction fragments.
    Methods Enzymol. 1980;65(1):75-85 PMID: 6154877
  14. Processing mechanisms in the biosynthesis of proteins.
    Ann N Y Acad Sci. 1980;343:1-16 PMID: 6994549
  15. Blood coagulation.
    Annu Rev Biochem. 1980;49:765-811 PMID: 6996572
  16. Plasmid screening at high colony density.
    Gene. 1980 Jun;10(1):63-7 PMID: 6997135
  17. Rat factor X is synthesized as a single chain precursor inducible by prothrombin fragments.
    J Biol Chem. 1982 Nov 10;257(21):13108-13 PMID: 7130196
  18. Isolation and characterization of a cDNA coding for human factor IX.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6461-4 PMID: 6959130
  19. Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX.
    Nucleic Acids Res. 1983 Apr 25;11(8):2325-35 PMID: 6687940
  20. Characterization of the complementary deoxyribonucleic acid and gene coding for human prothrombin.
    Biochemistry. 1983 Apr 26;22(9):2087-97 PMID: 6305407
  21. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  22. Kilo-sequencing: creation of an ordered nest of asymmetric deletions across a large target sequence carried on phage M13.
    Methods Enzymol. 1983;101:98-122 PMID: 6310345
  23. Biosynthesis of human preproapolipoprotein A-II.
    J Biol Chem. 1983 Nov 25;258(22):14054-9 PMID: 6315718
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.11.4481
Published
1984-06-11
Pages
4481-92
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318852
Subset
IM
Databases
GENBANK
X00673
External Links
PubMed source DOI: 10.1093/nar/12.11.4481 PMC Full Text View journal details
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