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PMID: 3092184 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Conservation of sequences adjacent to the telomeric C4A2 repeats of ciliate macronuclear ribosomal RNA gene molecules.

Nucleic acids research ·Vol. 14 ·No. 15 ·1986-08-11 ·Pages 6299-311

Challoner PB, Blackburn EH

Abstract

We sequenced and compared the telomeric regions of linear rDNAs from vegetative macronuclei of several ciliates in the suborder Tetrahymenina. All telomeres consisted of tandemly repeated C4A2 sequences, including the 5' telomere of the 11 kb rDNA from developing macronuclei of Tetrahymena thermophila. Our sequence of the 11 kb 5' telomeric region shows that each one of a previously described pair of inverted repeats flanking the micronuclear rDNA (Yao et al., Mol. Cell. Biol. 5: 1260-1267, 1985) is 29 bp away from the positions to which telomeric C4A2 repeats are joined to the ends of excised 11 kb rDNA. In general we found that the macronuclear rDNA sequences adjacent to C4A2 repeats are not highly conserved. However, in the non-palindromic rDNA of Glaucoma, we identified a single copy of a conserved sequence, repeated in inverted orientation in Tetrahymena spp., which all form palindromic rDNAs. We propose that this sequence is required for a step in rDNA excision common to both Tetrahymena and Glaucoma.

MeSH Terms
Animals Base Sequence Cell Nucleus/analysis Ciliophora/genetics,ultrastructure DNA, Recombinant/analysis DNA, Ribosomal/genetics RNA, Ribosomal/genetics Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Species Specificity Tetrahymena/genetics
Chemicals
DNA, Recombinant DNA, Ribosomal RNA, Ribosomal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Challoner P B
Blackburn E H
References (30)
30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.15.6299
Published
1986-08-11
Pages
6299-311
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311637
Subset
IM
Grants
NIGMS NIH HHS · GM 26259 · United States
NIGMS NIH HHS · GM 32565 · United States
Databases
GENBANK
X04204, X04205, X04206, X04207, X04208, X04209, X04210
External Links
PubMed source DOI: 10.1093/nar/14.15.6299 PMC Full Text View journal details
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