Strong sequence conservation of a 38 bp region near the center of the extrachromosomal rDNA palindrome in different Tetrahymena species.
The restriction-endonuclease map and the nucleotide sequence of the central region in the extrachromosomal rDNA palindrome of two micronuclear and one a-micronucleate species of Tetrahymena has been determined. The sequence data show that the different species investigated have a 24 or 26 nucleotide sequence region at the very center of the rDNA molecule which is non-palindromic. Comparison of the present sequence data with the published data of another micronucleate species reveal that a segment of 38 base pairs just outside the non-palindromic center is highly conserved in all the different species, while the rest of the central region show little sequence homology. The relevance of this conserved region to the amplification process of the rDNA molecule is discussed.
18 references, click to expand
-
Amplification of the rRNA genes in Tetrahymena.Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:1293-6 PMID: 290442
-
Extrachromosomal ribosomal RNA genes in Tetrahymena: structure and evolution.J Mol Biol. 1979 Nov 5;134(3):555-74 PMID: 537073
-
Sequencing end-labeled DNA with base-specific chemical cleavages.Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
-
Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.Gene. 1980 Jul;10(2):157-66 PMID: 6248420
-
Nucleotide sequence of the yeast plasmid.Nature. 1980 Aug 28;286(5776):860-5 PMID: 6251374
-
Detailed transcription map of the extrachromosomal ribosomal RNA genes in Tetrahymena thermophila.J Mol Biol. 1980 Sep 25;142(3):289-313 PMID: 6257908
-
Physical map of the ribosomal ribonucleic acid gene from Tetrahymena pyriformis.Biochemistry. 1981 Feb 17;20(4):905-9 PMID: 6260143
-
Extrachromosomal rDNA of Tetrahymena thermophila is not a perfect palindrome.Gene. 1981 Apr;13(3):281-7 PMID: 6266918
-
Replication of the extrachromosomal ribosomal RNA genes of Tetrahymena thermophilia.Nucleic Acids Res. 1981 Jul 24;9(14):3531-43 PMID: 7279667
-
Localization of putative transcription initiation site on the cloned rDNA fragment of Tetrahymena pyriformis.Nucleic Acids Res. 1981 Nov 25;9(22):5905-16 PMID: 6273812
-
Sequence homology near the center of the extrachromosomal rDNA palindrome in Tetrahymena.J Mol Biol. 1981 Dec 25;153(4):1151-5 PMID: 7343682
-
Unequal excision of complementary strands is involved in the generation of palindromic repetitions of rho- mitochondrial DNA in yeast.Cell. 1983 Feb;32(2):391-6 PMID: 6297793
-
Two separate regions of the extrachromosomal ribosomal deoxyribonucleic acid of Tetrahymena thermophila enable autonomous replication of plasmids in Saccharomyces cerevisiae.Mol Cell Biol. 1981 Jun;1(6):535-43 PMID: 6765606
-
An intervening sequence in the gene coding for 25S ribosomal RNA of Tetrahymena pigmentosa.Cell. 1979 Mar;16(3):565-73 PMID: 455443
-
Nascent subribosomal particles in Tetrahymena pyriformis.Eur J Biochem. 1970 Apr;13(2):238-46 PMID: 5439929
-
Free ribosomal DNA molecules from Tetrahymena pyriformis GL are giant palindromes.J Mol Biol. 1976 Jun 25;104(2):455-70 PMID: 820864
-
A single integrated gene for ribosomal RNA in a eucaryote, Tetrahymena pyriformis.Cell. 1977 Sep;12(1):121-32 PMID: 409501
-
The replication of ribosomal DNA in the macronucleus of Tetrahymena.Chromosoma. 1977 Dec 6;64(4):295-303 PMID: 413699
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