Home LiteratureArticle Details
PMID: 6765606 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two separate regions of the extrachromosomal ribosomal deoxyribonucleic acid of Tetrahymena thermophila enable autonomous replication of plasmids in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 1 ·No. 6 ·1981-06-00 ·Pages 535-43

Kiss GB, Amin AA, Pearlman RE

Abstract

Plasmids containing the nontranscribed central and terminal, but not the coding, regions of the extrachromosomal ribosomal deoxyribonucleic acid (rDNA) of Tetrahymena thermophila are capable of autonomous replication in Saccharomyces cerevisiae. These plasmids transform S. cerevisiae at high frequency; transformants are unstable in the absence of selection, and plasmids identical to those used for transformation were isolated from the transformed yeast cells. One plasmid contains a 1.85-kilobase Tetrahymena DNA fragment which includes the origin of bidirectional replication of the extrachromosomal rDNA. The other region of Tetrahymena rDNA allowing autonomous replication of plasmids in S. cerevisiae is a 650-base pair, adenine plus thymine-rich segment from the rDNA terminus. Neither of these Tetrahymena fragments shares obvious sequence homology with the origin of replication of the S. cerevisiae 2-microns circle plasmid or with ars1, an S. cerevisiae chromosomal replicator.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA Replication DNA, Ribosomal/genetics Molecular Sequence Data Plasmids Saccharomyces cerevisiae/genetics Tetrahymena/genetics
Chemicals
DNA, Ribosomal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kiss G B
Department of Biology, York University, Downsview, Ontario, Canada.
Amin A A
Pearlman R E
References (39)
39 references, click to expand
  1. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.
    Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21 PMID: 5231757
  2. Oligo(A)-stimulated Tetrahymena rDNA synthesis in vito.
    Can J Biochem. 1981 Jun;59(6):396-403 PMID: 6271358
  3. Replication and transcription of eukaryotic DNA in Escherichia coli.
    Proc Natl Acad Sci U S A. 1974 May;71(5):1743-7 PMID: 4600264
  4. Free ribosomal RNA genes in the macronucleus of Tetrahymena.
    Proc Natl Acad Sci U S A. 1974 Aug;71(8):3078-81 PMID: 4528573
  5. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.
    J Virol. 1974 Nov;14(5):1235-44 PMID: 4372397
  6. Specificity of substrate recognition by the EcoRI restriction endonuclease.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3310-4 PMID: 242001
  7. Isolation of yeast DNA.
    Methods Cell Biol. 1975;12:39-44 PMID: 1105072
  8. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  9. Palindromic base sequences and replication of eukaryote chromosome ends.
    Nature. 1974 Aug 9;250(5466):467-70 PMID: 4469597
  10. The macronuclear ribosomal DNA of Tetrahymena pyriformis is a palindrome.
    J Mol Biol. 1976 Jun 25;104(2):421-53 PMID: 820863
  11. Free ribosomal DNA molecules from Tetrahymena pyriformis GL are giant palindromes.
    J Mol Biol. 1976 Jun 25;104(2):455-70 PMID: 820864
  12. Isolation of bacteriophage lambda containing yeast ribosomal RNA genes: screening by in situ RNA hybridization to plaques.
    Cell. 1976 Jun;8(2):227-32 PMID: 786462
  13. Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit.
    Cell. 1976 Nov;9(3):431-8 PMID: 1033038
  14. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  15. DNA-DNA hybridization on membrane filters: a convenient method using formamide.
    Biochim Biophys Acta. 1977 Jul 15;477(2):191-4 PMID: 406929
  16. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  17. A model for switching on ribosomal RNA synthesis by creating a palindromic DNA sequence in the promoter region of the ribosomal RNA cistron: the "structon".
    J Theor Biol. 1977 Jun 7;66(3):573-82 PMID: 407400
  18. A single integrated gene for ribosomal RNA in a eucaryote, Tetrahymena pyriformis.
    Cell. 1977 Sep;12(1):121-32 PMID: 409501
  19. The replication of ribosomal DNA in the macronucleus of Tetrahymena.
    Chromosoma. 1977 Dec 6;64(4):295-303 PMID: 413699
  20. A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in Tetrahymena.
    J Mol Biol. 1978 Mar 25;120(1):33-53 PMID: 642006
  21. Transformation of yeast.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 PMID: 347451
  22. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  23. Transformation of yeast by a replicating hybrid plasmid.
    Nature. 1978 Sep 14;275(5676):104-9 PMID: 357984
  24. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 PMID: 375221
  25. An intervening sequence in the gene coding for 25S ribosomal RNA of Tetrahymena pigmentosa.
    Cell. 1979 Mar;16(3):565-73 PMID: 455443
  26. Synthesis of ribosomal DNA in conjugating Tetrahymena.
    Exp Cell Res. 1979 Oct 1;123(1):147-55 PMID: 488177
  27. Chimeric plasmids for cloning of deoxyribonucleic acid sequences in Saccharomyces cerevisiae.
    J Bacteriol. 1979 Oct;140(1):73-82 PMID: 387728
  28. Plasmid vehicles for direct cloning of Escherichia coli promoters.
    J Bacteriol. 1979 Nov;140(2):400-7 PMID: 387734
  29. Isolation and characterisation of a yeast chromosomal replicator.
    Nature. 1979 Nov 1;282(5734):39-43 PMID: 388229
  30. Replacement of chromosome segments with altered DNA sequences constructed in vitro.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4951-5 PMID: 388424
  31. Isolation of chromosomal origins of replication in yeast.
    Nature. 1980 Mar 13;284(5752):185-7 PMID: 6987527
  32. Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae.
    Nature. 1980 Apr 3;284(5755):426-30 PMID: 6987539
  33. Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.
    Gene. 1980 Jul;10(2):157-66 PMID: 6248420
  34. Nucleotide sequence of the yeast plasmid.
    Nature. 1980 Aug 28;286(5776):860-5 PMID: 6251374
  35. Eukaryotic DNA segments capable of autonomous replication in yeast.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4559-63 PMID: 6449009
  36. Construction and analysis of simian virus 40 origins defective in tumor antigen binding and DNA replication.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6491-5 PMID: 6256739
  37. Detailed transcription map of the extrachromosomal ribosomal RNA genes in Tetrahymena thermophila.
    J Mol Biol. 1980 Sep 25;142(3):289-313 PMID: 6257908
  38. Extrachromosomal rDNA of Tetrahymena thermophila is not a perfect palindrome.
    Gene. 1981 Apr;13(3):281-7 PMID: 6266918
  39. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.1.6.535-543.1981
Published
1981-06-00
Pages
535-43
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369696
Subset
IM
Databases
GENBANK
M10383
External Links
PubMed source DOI: 10.1128/mcb.1.6.535-543.1981 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2