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PMID: 3008087 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence characterization of Tetrahymena macronuclear DNA ends.

Nucleic acids research ·Vol. 14 ·No. 5 ·1986-03-11 ·Pages 2109-22

Yokoyama R, Yao MC

Abstract

Tetrahymena is a ciliated protozoan which has two nuclei: a micronucleus, which maintains the genetic continuity of the cell, and the macronucleus which is derived from the micronucleus after sexual conjugation. A macronuclear DNA library was constructed to contain DNA ends. A probe containing C4A2 repeats which are known to be present at macronuclear DNA ends (1) was used to screen the library. Three clones were characterized by sequencing, restriction enzyme mapping and Bal 31 digestion. The data indicate that these three clones represent macronuclear DNA ends which were generated by DNA fragmentation during macronuclear formation. The sequencing data at the C4A2 repeat junction show a conserved sequence of five nucleotides, TTATT. Sequences further away show no obvious homologies except that they are highly enriched in AT. This structure is quite different from the subtelomeric sequences of other organisms.

MeSH Terms
Animals Base Sequence Cell Nucleus/ultrastructure Chromosome Mapping Chromosomes/ultrastructure Cloning, Molecular DNA/genetics DNA Restriction Enzymes/metabolism Repetitive Sequences, Nucleic Acid Tetrahymena/genetics,ultrastructure
Chemicals
DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yokoyama R
Yao M C
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46 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.5.2109
Published
1986-03-11
Pages
2109-22
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339646
Subset
IM
Grants
NIGMS NIH HHS · GM26210 · United States
External Links
PubMed source DOI: 10.1093/nar/14.5.2109 PMC Full Text View journal details
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