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PMID: 6819543 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

The terminal organization of macronuclear DNA in Oxytricha fallax.

Nucleic acids research ·Vol. 10 ·No. 24 ·1982-12-20 ·Pages 8145-54

Pluta AF, Kaine BP, Spear BB

Abstract

The macronucleus of the hypotrichous ciliate Oxytricha fallax is transcriptionally active and contains linear achromosomal DNA molecules that function as single-gene units. The terminal organization of macronuclear DNA was analyzed by chemical sequencing and S1 mapping. The terminal sequence of total macronuclear DNA was determined for molecules labeled at the 5' or 3' ends. Results indicate that the 5' sequence C4A4C4A4C4 and the 3' sequence G4T4G4T4G4T4G4T4G4 occur at both ends of all DNA molecules in the macronucleus. The discrepancy in the length of the common terminal sequence between the 5' and 3' ends was clarified by a limited S1 digestion experiment, which indicated the existence of a 16 nucleotide long single-stranded tail at the 3' ends.

MeSH Terms
Animals Base Sequence Cell Nucleus/analysis Ciliophora/genetics DNA/genetics Genes Species Specificity
Chemicals
DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pluta A F
Kaine B P
Spear B B
References (27)
27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.24.8145
Published
1982-12-20
Pages
8145-54
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327075
Subset
IM
Grants
NIGMS NIH HHS · GM23440 · United States
External Links
PubMed source DOI: 10.1093/nar/10.24.8145 PMC Full Text View journal details
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