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PMID: 6776521 Published · ppublish English Journal Article

In vitro aggregation of the gene-sized DNA molecules of the ciliate Stylonychia mytilus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 7 ·1980-07-00 ·Pages 4104-7

Lipps HJ

Abstract

Macronuclear DNA of hypotrichous ciliates exists in the form of gene-sized DNA molecules. It can be resolved on agarose gels into a continuum of sizes upon which is imposed a set of characteristic DNA bands. Most or all of the DNA molecules carry identical terminal inverted repeat sequences. By incubating macronuclear DNA under increasingly stronger ionic conditions, high molecular weight DNA aggregates and ring-like DNA structures are formed. Experimental evidence is presented that this aggregation is not due to the presence of identical single-stranded DNA ends on each macronuclear DNA fragment, and an alternative model for DNA aggregation is discussed.

MeSH Terms
Animals Base Sequence Cell Nucleus/ultrastructure Cell-Free System Ciliophora/genetics DNA/genetics Genes Molecular Weight Nucleic Acid Renaturation Repetitive Sequences, Nucleic Acid
Chemicals
DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Lipps H J
References (24)
24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.7.4104
Published
1980-07-00
Pages
4104-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349778
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.7.4104 PMC Full Text View journal details
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