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PMID: 2987861 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Autonomously replicating sequences from the non transcribed spacers of Tetrahymena thermophila ribosomal DNA.

Nucleic acids research ·Vol. 13 ·No. 7 ·1985-04-11 ·Pages 2647-59

Amin AA, Pearlman RE

Abstract

The non-transcribed spacer (NTS) regions of the linear extrachromosomal palindromic rDNA from the ciliated protozoan Tetrahymena thermophila contain, in addition to sequences regulating transcription, the origin(s) of bidirectional replication as well as telomere associated sequences. These NTS regions function as autonomous replication sequences (ARS) in the yeast Saccharomyces cerevisiae (Kiss, G.B., Amin, A.A. and Pearlman, R.E., Mol. Cell Biol. 1: 535-543, 1981). We have now identified in the 5' NTS two adjacent but non-overlapping restriction fragments which function as ARS in yeast. These fragments contain 200 bp of duplicated sequence and include the in vivo origin of rDNA replication. The ARS in the 3' NTS has been subdivided into a sequence which allows high frequency transformation of yeast but with transformants extremely unstable when grown either under selective or non-selective conditions, and a sequence which appears to be required for plasmid maintenance yet not to be essential for high frequency transformation. Detailed analysis of the DNA sequences in these regions gives rise to interesting information about structural and functional features of the molecule.

MeSH Terms
Animals Computers DNA Restriction Enzymes/metabolism DNA, Recombinant/analysis DNA, Ribosomal/analysis Repetitive Sequences, Nucleic Acid Tetrahymena/genetics Transcription, Genetic
Chemicals
DNA, Recombinant DNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Amin A A
Pearlman R E
References (30)
30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.7.2647
Published
1985-04-11
Pages
2647-59
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341181
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.7.2647 PMC Full Text View journal details
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