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PMID: 3033433 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Peptide transport in Salmonella typhimurium: molecular cloning and characterization of the oligopeptide permease genes.

Molecular & general genetics : MGG ·Vol. 206 ·No. 1 ·1987-01-00 ·Pages 101-9

Hiles ID, Powell LM, Higgins CF

Abstract

The oligopeptide permease is encoded by at least four genes which are transcribed as a single operon. We cloned and characterized this operon from Salmonella typhimurium, as well as the flanking genes, tonB, ana and a new gene, cwd, which affects cell wall synthesis. We correlated the physical map of opp DNA with a detailed genetic map of the opp operon and the individual opp genes were accurately located with respect to various restriction sites by Southern blotting. The region of the chromosome near opp was found to be highly unstable with deletions arising at a highly frequency. The operon also contains hot-spots for IS1 and IS5 insertions.

MeSH Terms
Bacterial Proteins Chromosome Mapping Chromosomes, Bacterial/physiology Cloning, Molecular DNA Restriction Enzymes Genes Genes, Bacterial Membrane Transport Proteins/genetics Nucleic Acid Hybridization Operon Salmonella typhimurium/enzymology,genetics Transcription, Genetic
Chemicals
Bacterial Proteins Membrane Transport Proteins oligopeptide permease, Bacteria DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hiles I D
Powell L M
Higgins C F
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39 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00326543
Published
1987-01-00
Pages
101-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00326543
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