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PMID: 363691 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

HindII and HindIII restriction maps of the attphi80-tonB-trp region of the Escherichia coli genome, and location of the tonB gene.

Journal of bacteriology ·Vol. 136 ·No. 3 ·1978-12-00 ·Pages 1165-73

Postle K, Reznikoff WS

Abstract

The HindII and HindIII restriction maps of the attphi80-tonB-trp region of the Escherichia coli chromosome are presented. Analysis of phage DNAs carrying tonB mutations has allowed identification of a 1,730-base pair HindII fragment containing at least part of the tonB gene. This fragment is 4,020 base pairs from the end of trpA, with the total distance from attphi80 to trpA being 6,550 +/- 800 base pairs. Properties of hybrid plasmids containing insertions of various tonB+ restriction fragments suggest that tonB lies completely within the 1,730-base pair fragment. In addition, apparent fusions of beta-galactoside to proteins within the tonB region suggest that the entire region codes for more than one polypeptide.

MeSH Terms
Bacterial Proteins/biosynthesis Base Sequence Chromium/pharmacology Chromosome Mapping Chromosomes, Bacterial Colicins/pharmacology Coliphages/genetics DNA, Bacterial/genetics Escherichia coli/genetics Genes Plasmids Transduction, Genetic
Chemicals
Bacterial Proteins Colicins DNA, Bacterial Chromium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Postle K
Reznikoff W S
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.136.3.1165-1173.1978
Published
1978-12-00
Pages
1165-73
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218552
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.136.3.1165-1173.1978 PMC Full Text View journal details
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