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PMID: 6434517 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Anaerobic and leucine-dependent expression of a peptide transport gene in Salmonella typhimurium.

Journal of bacteriology ·Vol. 160 ·No. 1 ·1984-10-00 ·Pages 131-6

Jamieson DJ, Higgins CF

Abstract

Using Mu d1-mediated lac operon fusions, we studied the transcriptional regulation of the genes encoding two peptide transport systems, the oligopeptide permease and the tripeptide permease. The four opp genes were found to be constitutively expressed, whereas the genes encoding the tripeptide permease are under a complex set of regulatory controls. Two loci, tppA and tppB, are required for tripeptide permease function. Locus tppA is shown to be a positive regulator of tppB expression. In addition, tppB expression is specifically induced by exogeneous leucine or by anaerobiosis. Anaerobic induction of tppB is independent of the fnr gene product which is required for the anaerobic expression of several respiratory enzymes. Thus, there must be at least two distinct pathways for the anaerobic regulation of gene expression.

MeSH Terms
Anaerobiosis Bacterial Proteins Coliphages/genetics Genes Genes, Bacterial Genotype Lac Operon Leucine/pharmacology Membrane Transport Proteins/genetics Mutation Plasmids Salmonella typhimurium/enzymology,genetics Transcription, Genetic/drug effects beta-Galactosidase/genetics
Chemicals
Bacterial Proteins Membrane Transport Proteins oligopeptide permease, Bacteria beta-Galactosidase Leucine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jamieson D J
Higgins C F
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29 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.160.1.131-136.1984
Published
1984-10-00
Pages
131-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214691
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.160.1.131-136.1984 PMC Full Text View journal details
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