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PMID: 3027668 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Alternative splicing of SV40 early pre-mRNA in vitro.

Nucleic acids research ·Vol. 14 ·No. 24 ·1986-12-22 ·Pages 9911-26

van Santen VL, Spritz RA

Abstract

Simian virus 40 (SV40) early pre-mRNA is spliced using either of two alternative 5' splice sites and a common 3' splice site to produce two mRNAs that encode the T and t antigens. We have studied alternative splicing of SV40 early pre-mRNA in vitro using a HeLa cell nuclear extract. Synthetic SV40 early transcripts are processed to T and t antigen mRNAs in vitro. As in SV40-infected cells in vivo, cleavage at the T antigen 5' splice site is more efficient than cleavage at the t antigen 5' splice site in vitro, although both of these 5' splice sites are utilized relatively inefficiently in vitro. The ratio of cleavage at the T and t antigen 5' splice sites is not changed significantly by a number of alterations in the conditions under which the in vitro splicing reactions are carried out.

MeSH Terms
Animals Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/genetics Cell Nucleus/metabolism Female Genes Genes, Viral HeLa Cells/metabolism Humans Nucleic Acid Precursors/genetics Oncogene Proteins, Viral/genetics Oocytes/metabolism RNA Precursors RNA Splicing RNA, Messenger/genetics Simian virus 40/genetics Transcription, Genetic Xenopus
Chemicals
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Nucleic Acid Precursors Oncogene Proteins, Viral RNA Precursors RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
van Santen V L
Spritz R A
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30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.24.9911
Published
1986-12-22
Pages
9911-26
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341343
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.24.9911 PMC Full Text View journal details
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