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PMID: 3027396 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Germ line integration of a murine leukemia provirus into a retroviruslike sequence.

Journal of virology ·Vol. 61 ·No. 3 ·1987-03-00 ·Pages 701-7

Horowitz JM, Holland GD, King SR, Risser R

Abstract

Nucleotide sequence analysis of the cellular sequences flanking the integrated ecotropic (mouse-infectious) murine leukemia provirus of BALB/c mice indicated that the murine leukemia provirus is integrated in opposing transcriptional orientation within a solo long terminal repeat (LTR) of the VL30 family of endogenous retrovirus-related sequences. To quantify the effect of this integration event on the ability of the ecotropic provirus to be expressed, we constructed recombinant molecules that carried the chloramphenicol acetyltransferase (cat) gene and various viral LTRs and determined the CAT activity induced by these constructs after transfection of NIH 3T3 cells. Our results indicate that the BALB/c ecotropic LTR is about 10-fold more active than the VL30 LTR. The presence of the VL30 LTR did not affect the transcriptional activity of the ecotropic LTR in the context of the integration event. Our results also indicate that the LTRs of the BALB/c provirus are less transcriptionally active than are the proviral LTRs of AKR murine leukemia virus and the Harvey murine sarcoma virus.

MeSH Terms
Animals Cell Transformation, Viral DNA, Viral/genetics Enhancer Elements, Genetic Gene Expression Regulation Leukemia Virus, Murine/genetics Mice Mice, Inbred AKR Mice, Inbred BALB C Recombination, Genetic Repetitive Sequences, Nucleic Acid Transcription, Genetic
Chemicals
DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Horowitz J M
Holland G D
King S R
Risser R
References (43)
43 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.3.701-707.1987
Published
1987-03-00
Pages
701-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254009
Subset
IM
Grants
NCI NIH HHS · CA07175 · United States
NCI NIH HHS · CA09135 · United States
NCI NIH HHS · CA22443 · United States
Databases
GENBANK
M15425
External Links
PubMed source DOI: 10.1128/JVI.61.3.701-707.1987 PMC Full Text View journal details
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